Developmental regulation of GLUT-1 (erythroid/Hep G2) and GLUT-4 (muscle/fat) glucose transporter expression in rat heart, skeletal muscle, and brown adipose tissue.
Developmental regulation of GLUT-1 (erythroid/Hep G2) and GLUT-4 (muscle/fat) glucose transporter expression in rat heart, skeletal muscle, and brown adipose tissue.
复制标题
大鼠心脏、骨骼肌和棕色脂肪组织中 GLUT-1（红细胞/Hep G2）和 GLUT-4（肌肉/脂肪）葡萄糖转运蛋白表达的发育调节。
DOI：
10.1210/endo.130.2.1370797
复制
发表时间：
1992
期刊：
影响因子：
4.8
通讯作者：
A. Zorzano
中科院分区：
文献类型：
--
作者：
T. Santalucía;M. Camps;Anna Castelló;P. Muñoz;A. Nuel;X. Testar;M. Palacín;A. Zorzano
在大鼠心脏、骨骼肌和棕色脂肪组织发育过程中，评估了GLUT-1（红细胞/Hep G2）和GLUT-4（肌肉/脂肪）葡萄糖转运蛋白的表达。GLUT-4蛋白的表达在妊娠第21天的胎儿心脏中可检测到，出生后逐渐增加，在纳塔尔后第15天达到接近成人的水平。相比之下，GLUT-4信使RNA（mRNA）已经存在于17日龄胎儿的心脏中。出生后早期，心脏和棕色脂肪组织中的GLUT-4 mRNA保持较低水平，仅在纳塔尔后第10天后增加。GLUT-4蛋白在骨骼肌发育过程中的表达模式与在心脏中观察到的相似。与心脏和骨骼肌相反，在胎儿晚期，棕色脂肪组织中检测到高水平的GLUT-4蛋白（成人的30%）。在胎儿期，GLUT-1在棕色脂肪组织、心脏和骨骼肌中呈现非常高的表达水平。出生后不久，GLUT-1蛋白逐渐减少，在心脏和骨骼肌中在第10天达到成人水平。心脏中的GLUT-1 mRNA水平遵循与GLUT-1蛋白相似的模式，在胎儿期非常高，在纳塔尔后早期降低。GLUT-1蛋白在棕色脂肪组织中表现出复杂的模式：胎儿水平较高，出生后下降，随后在纳塔尔后增加，在第9天达到峰值。孕激素诱导的成熟后保护骨骼肌和棕色脂肪组织中与纳塔尔后生活相关的GLUT-1蛋白的减少。然而，GLUT-4的诱导并没有被阻止过成熟的任何组织进行研究。这些结果表明：1）在胎儿和纳塔尔后早期，GLUT-1是在心脏、骨骼肌和棕色脂肪组织中表达的主要葡萄糖转运蛋白同种型; 2）在纳塔尔后早期，存在广泛的GLUT-1抑制; 3）在发育期间，GLUT-1的蛋白质和mRNA水平之间存在密切相关性，因此在翻译前水平的调节起主要调节作用; 4）GLUT-4蛋白诱导的开始发生在胎儿生命的第20-21天之间;基于在大鼠心脏和棕色脂肪组织中获得的数据，在发育期间GLUT-4的mRNA和蛋白水平之间存在解离，表明在翻译或翻译后步骤的修饰;和5）出生后不久观察到的过熟阻断了GLUT-1表达的减少，但不阻断GLUT-4的诱导。（400字处截断摘要）
The expression of GLUT-1 (erythroid/Hep G2) and GLUT-4 (muscle/fat) glucose transporters was assessed during development in rat heart, skeletal muscle, and brown adipose tissue. GLUT-4 protein expression was detectable in fetal heart by day 21 of pregnancy; it increased progressively after birth, attaining levels close to those of adults at day 15 post natal. In contrast, GLUT-4 messenger RNA (mRNA) was already present in hearts from 17 day-old fetuses. GLUT-4 mRNA stayed low during early postnatal life in heart and brown adipose tissue and only increased after day 10 post natal. The expression pattern for GLUT-4 protein in skeletal muscle during development was comparable to that observed in heart. In contrast to heart and skeletal muscle, GLUT-4 protein in brown adipose tissue was detected in high levels (30% of adult) during late fetal life. During fetal life, GLUT-1 presented a very high expression level in brown adipose tissue, heart, and skeletal muscle. Soon after birth, GLUT-1 protein diminished progressively, attaining adult levels at day 10 in heart and skeletal muscle. GLUT-1 mRNA levels in heart followed a similar pattern to the GLUT-1 protein, being very high during fetal life and decreasing early in post natal life. GLUT-1 protein showed a complex pattern in brown adipose tissue: fetal levels were high, decreased after birth, and increased subsequently in post natal life, reaching a peak by day 9. Progesterone-induced postmaturity protected against the decrease in GLUT-1 protein associated with post natal life in skeletal muscle and brown adipose tissue. However, GLUT-4 induction was not blocked by postmaturity in any of the tissues subjected to study. These results indicate that: 1) during fetal and early post natal life, GLUT-1 is a predominant glucose transporter isotype expressed in heart, skeletal muscle, and brown adipose tissue; 2) during early post natal life there is a generalized GLUT-1 repression; 3) during development, there is a close correlation between protein and mRNA levels for GLUT-1, and therefore regulation at a pretranslational level plays a major regulatory role; 4) the onset of GLUT-4 protein induction occurs between days 20-21 of fetal life; based on data obtained in rat heart and brown adipose tissue, there is a dissociation during development between mRNA and protein levels for GLUT-4, suggesting modifications at translational or posttranslational steps; and 5) postmaturity blocks the decrease in GLUT-1 expression but not the induction of GLUT-4, observed soon after birth.(ABSTRACT TRUNCATED AT 400 WORDS)
登录
查看更多内容
DOI：
10.1016/s0021-9258(19)37577-5
发表时间：
1988-10
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
T. Kayano;H. Fukumoto;R. Eddy;Y. Fan;M. Byers;T. Shows;G. Bell
通讯作者：
T. Kayano;H. Fukumoto;R. Eddy;Y. Fan;M. Byers;T. Shows;G. Bell
胰岛素对心肌葡萄糖转运的作用。
DOI：
--
发表时间：
1984
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Watanabe,T;Smith,MM;Robinson,FW;Kono,T
通讯作者：
Kono,T
DOI：
10.1016/s0021-9258(18)77362-6
发表时间：
1990-08
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
A. Douen;T. Ramlal;Sanjay Rastogi;P. Bilan;G. Cartee;M. Vranic;J. Holloszy;A. Klip
通讯作者：
A. Douen;T. Ramlal;Sanjay Rastogi;P. Bilan;G. Cartee;M. Vranic;J. Holloszy;A. Klip
3T3-L1 脂肪细胞中胰岛素响应性己糖转运的增加与新型转运蛋白基因的表达相关。
DOI：
--
发表时间：
1989
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
GarciadeHerreros,A;Birnbaum,MJ
通讯作者：
Birnbaum,MJ
D-葡萄糖转运蛋白是组织特异性的。
DOI：
--
发表时间：
1987
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Wang,C
通讯作者：
Wang,C