Seasonal differential expression of KiSS-1/GPR54 in the striped hamsters (Cricetulus barabensis) among different tissues
Seasonal differential expression of KiSS-1/GPR54 in the striped hamsters (Cricetulus barabensis) among different tissues
复制标题
条纹仓鼠(Cricetulus barabensis)不同组织中KiSS-1/GPR54的季节性差异表达
DOI：
10.1111/1749-4877.12223
复制
发表时间：
2017
影响因子：
3.3
通讯作者：
Chen Lei
中科院分区：
文献类型：
--
作者：
Xu Laixiang;Xue Huiliang;Li Shenning;Xu Jinhui;Chen Lei
Kispeptins和G蛋白偶联受体(GPR54)在季节性繁殖动物的繁殖活动中起着重要的调节作用，但KiSS-1和GPR54基因影响条纹仓鼠季节性繁殖的机制尚不清楚。本研究采用实时荧光定量聚合酶链式反应技术，检测了4个不同季节下丘脑、卵巢、睾丸、子宫和附睾中KiSS-1和GPR54的表达谱。结果表明，在不同季节，雄性条纹仓鼠的Kiss-1表达模式和雌性条纹仓鼠GPR54的表达模式与下丘脑的季节性光周期一致。同时，在不同季节，雄性条纹仓鼠睾丸中KiSS-1的表达谱和下丘脑中GPR54的表达谱与其季节性繁殖活动的强度一致。在不同组织中，GPR54的表达趋势是四季一致的，而KiSS-1的表达趋势是组织依赖的。GPR54在4个季节的表达趋势是一致的，而KiSS-1的表达趋势在不同的季节差异很大，并且具有性别依赖性。这些结果表明，KiSS-1和GPR54在条纹仓鼠中的表达受到复杂机制的调节，并且这种调节机制具有季节性和性别依赖性。本研究将为研究KiSS-1和GPR54对季节繁殖的影响及其机制提供理论依据。
Kisspeptins and G protein coupled receptor (GPR54) play significant roles in regulating reproductive activity among seasonally reproductive animals; however, the mechanisms ofKiSS‐1andGPR54gene affecting the seasonal reproduction in striped hamster are still unknown. In this study, real‐time quantitative polymerase chain reaction was employed to examine the expression profiles ofKiSS‐1andGPR54in the hypothalamus, ovaries, testes, uterus and epididymis of striped hamsters across 4 different seasons. Our results showed that, across different seasons, theKiSS‐1expression mode of male striped hamsters and theGPR54expression mode of female striped hamsters were consistent with the seasonal photoperiod in the hypothalamus. Meanwhile, across different seasons, the expression profile ofKiSS‐1in the testes and theGPR54expression profile of male striped hamsters in the hypothalamus were consistent with the intensity of their seasonal reproductive activity. Among different tissues, the expression trend forGPR54is consistent across 4 seasons, while that forKiSS‐1is tissue‐dependent. The expression trend forGPR54across 4 seasons is the same regardless of gender, while that forKiSS‐1is dramatically different and sex‐dependent across different seasons. These results suggest that the expressions ofKiSS‐1andGPR54in the striped hamsters were regulated by complicated mechanisms, and the regulatory mechanisms in the striped hamsters are seasonal‐dependent and sex‐dependent. This research will provide a theoretical basis for studying howKiSS‐1andGPR54affect seasonal reproduction and the mechanisms behind their influence.