Developmental timing of hair follicle and dorsal skin innervation in mice
Developmental timing of hair follicle and dorsal skin innervation in mice
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DOI：
10.1002/cne.10212
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发表时间：
2002-06-17
影响因子：
2.5
通讯作者：
Paus, R
中科院分区：
文献类型：
--
作者：
Peters, EMJ;Botchkarev, VA;Paus, R
毛囊的神经支配为剖析皮肤形态发生过程中的逐步神经支配提供了一个有趣但鲜有研究的模型。我们使用免疫荧光和一组神经元标记物来描述C57BL/6小鼠背部皮肤神经支配的发育过程。小鼠皮肤神经支配的发育是连续进行的。在胚胎第15天（E15），在毛囊发育的任何形态学证据出现之前，第一批皮肤神经纤维就已出现。在E16时，处于1期和2期发育的毛囊已经与神经纤维相关联。这些纤维靠近一个位置，在发育后期，毛囊（神经）网络A（FNA）就位于完全发育的被毛毛囊上。在出生前（E18），一些神经纤维已经穿透表皮，另外一组毛囊周围神经纤维围绕5期毛囊的峡部和隆突区域排列，发育成毛囊（神经）网络B（FNB）。到出生当天（P1），神经肽P物质和降钙素基因相关肽首先在皮下和真皮神经纤维中可被检测到。E18和P1新形成的毛囊显示出与第一批毛囊发育中相同的神经支配模式。就在毛干穿透表皮之前（P5），肽组氨酸甲硫氨酸免疫反应性神经纤维可被检测到，表皮神经支配达到峰值；这种神经支配在穿透后（P7 - P17）减少。最后，酪氨酸羟化酶免疫反应性和神经肽Y免疫反应性很容易被检测到。这种神经支配发育的顺序与毛囊发育始终相关，表明神经元和上皮形态发生密切相互依赖。
The innervation of hair follicles offers an intriguing, yet hardly studied model for the dissection of the stepwise innervation during cutaneous morphogenesis. We have used immunofluorescence and a panel of neuronal markers to characterize the developmental choreography of C57BL/6 mouse backskin innervation. The development of murine skin innervation occurs in successive waves. The first cutaneous nerve fibers appeared before any morphological evidence of hair follicle development at embryonic day 15 (E15). Stage 1 and 2 developing hair follicles were already associated with nerve fibers at E16. These fibers approached a location where later in development the follicular (neural) network A (FNA) is located on fully developed pelage hair follicles. Prior to birth (E18), some nerve fibers had penetrated the epidermis, and an additional set of perifollicular nerve fibers arranged itself around the isthmus and bulge region of stage 5 hair follicles, to develop into the follicular (neural) network B (FNB). By the day of birth (P1), the neuropeptides substance P and calcitonin gene-related peptide became detectable in subcutaneous and dermal nerve fibers first. Newly formed hair follicles on E18 and P1 displayed the same innervation pattern seen in the first wave of hair follicle development. Just prior to epidermal penetration of hair shafts (P5), peptide histidine methionine-IR nerve fibers became detectable and epidermal innervation peaked; such innervation decreased after penetration (P7- P17). Last, tyrosine hydroxylase-IR and neuropeptide Y-IR became readily detectable. This sequence of developing innervation consistently correlates with hair follicle development, indicating a close interdependence of neuronal and epithelial morphogenesis.