Morphological and genetic differences of Trypanosoma in some Chinese freshwater fishes:: difficulties of species identification
Morphological and genetic differences of Trypanosoma in some Chinese freshwater fishes:: difficulties of species identification
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DOI：
10.1007/s00436-007-0536-5
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发表时间：
2007-08-01
影响因子：
2
通讯作者：
Gong, Xiaoning
中科院分区：
文献类型：
--
作者：
Gu, Zemao;Wang, Jianguo;Gong, Xiaoning
对采自湖北牛山湖的黄Web和鲤鱼的血液涂片和纯化的锥虫进行了检测，以确定它们是否都是一种寄主特异性的、广泛存在于中国地区的伪锥虫。16份血液涂片中检出锥虫16份，形态计量学分析表明，锥虫有3个形态种：卡氏锥虫、假巴氏锥虫和锥虫。从16个样品的18S rDNA序列中可以看出，这些鱼的锥体有3个遗传群体。组1来自鲤鱼，组2含有梭鱼；组3来自黄褐孔菌，组3分别含有梭子虫T.sp.Pseudobagri和T.sp.形态特征和18S rDNA序列高度相似，表明鲤鱼和青鱼可能是同一种。假木霉为中国的首次发现。序列比较表明，T.spPartiobagri序列与无性系Marv最为相似，而T.sp序列与T.spcarpio和T.spPartiobagri的序列差异分别为5.4%和5.8%，初步鉴定为T.posobagri。结果表明，牛山湖鱼类中存在3种锥虫，至少存在3种不同的基因型别，而该地区的黄斑锥虫似乎同时含有这两种类型，但对假锥虫的鉴定仍是个问题。
Blood smears and purified trypanosome from freshwater fishes yellow catfish (Pseudobagras fulvidraco) and common carp (Cyprinus carpio) captured from Niushan Lake, Hubei Province were examined to determine whether all of their trypanosomes were Trypanosoma pseudobagri, a species of supposed host specificity and widespread existence across China. Trypanosomes occurred in 16/16 blood smears, and morphometric character analysis of trypanosomes from these smears showed that there were three morphospecies, Trypanosoma sp Carpio, T. sp Pseudobagri, and T. sp. 18S rDNA sequences of trypanosomes from 16 samples revealed three genetic groups among these fish trypanosomes. Group 1 was from C. carpio containing T. sp Carpio; groups 2 and 3 were from P. fulvidraco containing T. sp Pseudobagri and T. sp, respectively. The high similarity of morphometric characters and 18S rDNA sequences showed that T. sp Carpio and T. siniperca probably were the same species. T. sp Pseudobagri was the first occurrence in China. Sequence comparison showed that T. sp Pseudobagri sequence was most similar to that of clone Marv, whereas T. sp sequence differ from those of T. sp Carpio and T. sp Pseudobagri by 5.4 and 5.8%, respectively, and tentatively identified as T. pseudobagri. It was concluded that three species of trypanosomes, at least three genotypes occur in Niushan Lake fishes, and P. fulvidraco in this region appear to contain both types, although the identification of T. pseudobagri remains a problem.