Expression of toll-like receptors 2, 3, 4, and 9 genes in the human endometrium during the menstrual cycle
Expression of toll-like receptors 2, 3, 4, and 9 genes in the human endometrium during the menstrual cycle
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DOI：
10.1016/j.jri.2006.11.004
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发表时间：
2007-06-01
影响因子：
3.4
通讯作者：
Taketani, Yuji
中科院分区：
文献类型：
--
作者：
Hirata, Tetsuya;Osuga, Yutaka;Taketani, Yuji
子宫内膜的天然免疫对生殖具有重要意义。尽管Toll样受体（TLR）在先天性免疫应答中起着重要作用，但其在人子宫内膜中的表达仍有待充分阐明。我们已经研究了TLR 2，TLR 3，TLR 4和TLR 9在子宫内膜组织中的基因表达的实时定量PCR和原位杂交。子宫内膜组织中的四个基因的表达水平在月经周期中以相似的模式变化;水平在围月经期高，在排卵期低。在整个月经周期的上皮细胞和基质细胞中检测到这四个基因的表达。上皮细胞中的TLR 3和基质细胞中的TLR 4的表达水平较高，而上皮细胞和基质细胞中的TLR 2和TLR 9的表达水平相当。这些发现意味着TLRs的不同时空表达模式有助于子宫内膜的固有免疫。(C)2006爱思唯尔爱尔兰有限公司保留所有权利。
Innate immunity in the endometrium has fundamental significance for reproduction. Although toll-like receptors (TLRs) play central roles in innate immune responses, their expression in the human endometrium remains to be fully elucidated. We have examined the gene expression of TLR2, TLR3, TLR4, and TLR9 in endometrial tissues by real-time quantitative PCR and in situ hybridization. The expression levels of the four genes in endometrial tissues varied in a similar pattern during the menstrual cycle; the levels were high in the perimenstrual period and low in the periovulatory period. Expression of the four genes was detected in both epithelial cells and stromal cells throughout the menstrual cycle. Expression levels were higher in epithelial cells for TLR3 and in stromal cells for TLR4, while they were comparable in epithelia] cells and stromal cells for TLR2 and TLR9. These findings imply that differential spatio-temporal expression patterns of TLRs subserve proper innate immunity of the endometrium. (C) 2006 Elsevier Ireland Ltd. All rights reserved.