Vasopressin depolymerizes apical F-actin in rat inner medullary collecting duct.
Vasopressin depolymerizes apical F-actin in rat inner medullary collecting duct.
复制标题
加压素解聚大鼠内髓集合管中的顶端 F-肌动蛋白。
DOI：
10.1152/ajpcell.1993.265.3.c757
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
Hays,RM
中科院分区：
文献类型：
--
作者：
Simon,H;Gao,Y;Franki,N;Hays,RM
在两栖动物膀胱中，精氨酸加压素（AVP）解聚颗粒细胞顶端区域的F-肌动蛋白，促进水通道携带囊泡与顶端膜的融合。我们现在报告AVP对大鼠内髓集合管中段和终末段（IMCD 2和IMCD 3）F-肌动蛋白的影响。在IMCD 3中，在采用罗丹明-鬼笔环肽结合技术的全细胞测定中，2.5-250 nM AVP刺激5分钟显著解聚F-肌动蛋白13-24%。顶CD 2更敏感，对亚纳摩尔（0.25 nM）AVP的反应为6 +/- 2%解聚。解聚发生早在2.5和25 nM AVP后2分钟，但不是250 nM AVP。8-2.5 nM AVP使IMCD 3主细胞顶端肌动蛋白池的免疫金标记减少了26 +/- 5%（P < 0.05）;侧池和基底池无显著变化。血管内皮细胞、Henle薄肢和闰间细胞的免疫金标记在AVP后无变化。因此，在哺乳动物和两栖动物靶细胞中，AVP对顶端肌动蛋白网络的重组是一致的。
In amphibian bladder, arginine vasopressin (AVP) depolymerizes F-actin in the apical region of the granular cell, promoting fusion of water channel-carrying vesicles with the apical membrane. We now report the effect of AVP on F-actin in the mid- and terminal segments of rat inner medullary collecting duct (IMCD2 and IMCD3). In IMCD3, 5 min of stimulation by 2.5-250 nM AVP significantly depolymerized F-actin by 13-24% in whole cell assays employing the rhodamine-phalloidin binding technique. The IMCD2 was more sensitive, responding to subnanomolar (0.25 nM) AVP with 6 +/- 2% depolymerization. Depolymerization occurred as early as 2 min after 2.5 and 25 nM but not 250 nM AVP. 8-Bromoadenosine 3',5'-cyclic monophosphate depolymerized F-actin in IMCD3 at both 2 and 5 min. Immunogold labeling of the apical actin pool in IMCD3 principal cells was reduced by 26 +/- 5% (P < 0.05) by 2.5 nM AVP; the lateral and basal pools showed no significant changes. Capillary endothelial, thin limb of Henle, and intercalated cells showed no changes in immunogold labeling after AVP. Thus reorganization of the apical actin network by AVP is a consistent finding in both mammalian and amphibian target cells.