Dried blood spots versus plasma for the quantification of HIV-1 RNA using the manual (PCR-ELISA) amplicor monitor HIV-1 version 1.5 assay in Yaounde, Cameroon.
Dried blood spots versus plasma for the quantification of HIV-1 RNA using the manual (PCR-ELISA) amplicor monitor HIV-1 version 1.5 assay in Yaounde, Cameroon.
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DOI：
10.1177/1545109709333111
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发表时间：
2009-05-01
期刊：
影响因子：
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通讯作者：
Ndumbe, P M
中科院分区：
文献类型：
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作者：
Ikomey, G M;Atashili, J;Ndumbe, P M
目的：考虑到最近在资源有限的环境中对病毒载量定量的需求，本研究评估了使用干血斑 (DBS) 与血浆作为样本收集和储存的方法，使用非自动测定进行 HIV-1 RNA 定量。方法：从 60 名同意的 HIV-1 阳性患者中采集静脉血，在 4 小时内分离血浆，并储存在 -20 摄氏度。静脉血，50 microL，被吸干在 Whatman 滤纸的 4 个指定区域上，并在室温下风干 2 小时。 结果：使用血浆和 DBS 的 HIV-1 RNA 病毒载量之间存在很强的统计显着相关性 (r = .955，P < .001)。平均血浆病毒载量仅略高于 DBS 病毒载量（平均差异：0.06 log(10) 拷贝/mL）。结论：即使使用完全手动的 HIV 定量测定，DBS 也可以为资源有限环境中的 HIV-1 RNA 定量样品采集和储存提供可靠、经济有效的方法。
OBJECTIVES: Considering the recent accrued need for viral load quantification in resource-limited settings, this study evaluated the use of dried blood spots (DBS) compared to plasma as a means of sample collection and storage for HIV-1 RNA quantification using a non-automated assay.METHODS: Venous blood was collected from 60 consenting HIV-1-positive patients, plasma separated within 4 hours, and stored at -20 degrees C. Venous blood, 50 microL, was blotted on 4 designated areas of Whatman filter paper and air-dried at room temperature for 2 hours.RESULTS: There was a strong statistically significant correlation between HIV-1 RNA viral load using plasma and DBS (r = .955, P < .001). On average plasma viral loads were only slightly higher than DBS viral loads (mean difference: 0.06 log(10) copies/mL).CONCLUSION: Even when using an entirely manual HIV-quantification assay, DBS may provide a reliable, cost-effective method for sample collection and storage for HIV-1 RNA quantification in resource-limited settings.