Noble Metal Coated Single-Walled Carbon Nanotubes for Applications in Surface Enhanced Raman Scattering Imaging and Photothermal Therapy
Noble Metal Coated Single-Walled Carbon Nanotubes for Applications in Surface Enhanced Raman Scattering Imaging and Photothermal Therapy
复制标题
用于表面增强拉曼散射成像和光热治疗的贵金属涂层单壁碳纳米管
DOI：
10.1021/ja300140c
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发表时间：
2012-05-02
影响因子：
15
通讯作者：
Liu, Zhuang
中科院分区：
文献类型：
--
作者：
Wang, Xiaojing;Wang, Chao;Liu, Zhuang
具有各种独特光学性质的单壁碳纳米管（SWNT）是在生物医学成像和光疗中广泛探索的有趣纳米探针。本文中，DNA官能化的SWNT通过原位溶液相合成方法用贵金属（Ag或Au）纳米颗粒修饰，所述原位溶液相合成方法包括种子附着、种子生长和用聚乙二醇（PEG）进行表面修饰;产生在生理环境中稳定的SWNT-Ag-PEG和SWNT-Au-PEG纳米复合材料。在单壁碳纳米管-金属纳米复合物表面修饰金或银纳米粒子，可获得良好的浓度和激发源依赖的表面增强拉曼散射（Sers）效应。采用近红外（NIR）激光作为激发源，对叶酸（FA）标记的单壁碳纳米管-金纳米复合物标记的癌细胞进行靶向拉曼成像（SWNT-Au-PEG-FA）的实现，与使用非增强SWNT拉曼探针相比，在显着缩短的时间内获得的图像。由于由金壳贡献的强表面等离子体共振吸收，SWNTs-Au-PEG-FA纳米复合物还提供了显著改善的光热癌细胞杀伤功效。本工作提出了一种简便的方法来合成水溶性贵金属涂层的单壁碳纳米管，具有很强的Sers效应，适用于生物样品的标记和快速拉曼光谱成像，这是以前很少实现。因此，这里开发的SWNT-Au-PEG纳米复合材料可能是一种有趣的光学治疗诊断探针，用于癌症成像和治疗。
Single-walled carbon nanotubes (SWNTs) with various unique optical properties are interesting nanoprobes widely explored in biomedical imaging and phototherapies. Herein, DNA-functionalized SWNTs are modified with noble metal (Ag or Au) nanoparticles via an in situ solution phase synthesis method comprised of seed attachment, seeded growth, and surface modification with polyethylene glycol (PEG); yielding SWNT-Ag-PEG and SWNT-Au-PEG nanocomposites stable in physiological environments. With gold or silver nanoparticles decorated on the surface, the SWNT-metal nanocomposites gain an excellent concentration and excitation-source dependent surface-enhanced Raman scattering (SERS) effect Using a near-infrared (NIR) laser as the excitation source, targeted Raman imaging of cancer cells labeled with folic acid (FA) conjugated SWNT-Au nanocomposite (SWNT-Au-PEG-FA) is realized, with images acquired in significantly shortened periods of time as compared to that of using nonenhanced SWNT Raman probes. Owing to the strong surface plasmon resonance absorption contributed by the gold shell, the SWNTs-Au-PEG-FA nanocomposite also offers remarkably improved photothermal cancer cell killing efficacy. This work presents a facile approach to synthesize water-soluble noble metal coated SWNTs with a strong SERS effect suitable for labeling and fast Raman spectroscopic imaging of biological samples, which has been rarely realized before. The SWNT-Au-PEG nanocomposite developed here may thus be an interesting optical theranostic probe for cancer imaging and therapy.