Advanced flowcytometric analysis of regulatory T cells: CD127 downregulation early post stem cell transplantation and altered Treg/CD3+CD4+-ratio in severe GvHD or relapse
Advanced flowcytometric analysis of regulatory T cells: CD127 downregulation early post stem cell transplantation and altered Treg/CD3+CD4+-ratio in severe GvHD or relapse
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DOI：
10.1016/j.jim.2011.07.018
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发表时间：
2011-10-28
影响因子：
2.2
通讯作者：
Koehl, Ulrike
中科院分区：
文献类型：
--
作者：
Bremm, Melanie;Huenecke, Sabine;Koehl, Ulrike
调节性T细胞（Tcells，Tcells）对同种异体干细胞移植（SCT）后抑制移植物抗宿主病（GvHD）至关重要，但也已知会损害抗肿瘤免疫。为了研究Treg在GvHD或复发中的百分比和绝对数量的关系，我们在SCT后的前两年中检测了16例患者的239个外周血（PB）样本中的CD 4（+）CD 25（+/hi）CD 127（lo/-）Treg。建立了一个10色流式细胞仪面板来评估Treg亚群，并在10个健康个体中进行了测试。在患者中，我们证明了SCT后早期T细胞上的CD 127表达减少，并在第一年中增加。而且。在GvHD患者中，TvD达到更高的绝对数
Regulatory T cells (Tregs) are of crucial importance to suppress graft versus host disease (GvHD) post allogeneic stem cell transplantation (SCT), but are also known to impair antitumor immunity. However, Treg longitudinal studies are rare and in this respect advanced flowcytometric approaches for Treg characterization are necessary.To investigate the relation of both the percentage and the absolute numbers of Tregs on GvHD or relapse we measured CD4(+)CD25(+/hi)CD127(lo/-) Tregs in 239 peripheral blood (PB) samples of 16 patients during the first two years post-SCT. A 10-color flowcytometric panel was established to evaluate Treg subpopulations and has been tested in ten healthy individuals.In patients we demonstrated a decrease in CD127 expression on T cells early post-SCT which increases during the first year. Moreover. Tregs reached higher absolute numbers in patients with GvHD