Isolation and Purification of Primary Schwann Cells
Isolation and Purification of Primary Schwann Cells
复制标题
DOI：
10.1002/0471142301.ns0317s08
复制
发表时间：
1999-08
影响因子：
--
通讯作者：
D. E. Weinstein;R. Wu
中科院分区：
文献类型：
--
作者：
D. E. Weinstein;R. Wu
雪旺细胞是周围神经系统（PNS）的神经胶质细胞，其为其相关轴突提供髓鞘和营养支持。它们的功能对于PNS的正常发育、稳态和再生至关重要。可以分离许旺细胞并在培养物中扩增。这种培养物可用作培养物中髓鞘生成神经胶质的来源或用作各种细胞和分子生物学实验的许旺细胞的来源。这些细胞的增殖能力在这里提出的分离程序中被利用。
Schwann cells are the glial cells of the peripheral nervous system (PNS) which both myelinate and provide trophic support for their associated axons. Their functions are critical for proper development, homeostasis, and regeneration of the PNS. Schwann cells can be isolated and expanded in culture. Such a culture can be used as a source of myelinating glia in culture or as a source of Schwann cells for various cell and molecular biological experiments. The proliferative ability of these cells is exploited in the isolation procedures presented here.