Some aspects of porphyrin neurotoxicity in vitro.
Some aspects of porphyrin neurotoxicity in vitro.
复制标题
卟啉体外神经毒性的某些方面。
DOI：
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发表时间：
1982
影响因子：
2.1
通讯作者：
J. Kennedy
中科院分区：
文献类型：
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作者：
R. Riopelle;J. Kennedy
使用分离的初级感觉神经元培养系统，观察到一些天然存在的卟啉产生剂量依赖性神经毒性，如通过神经元死亡和通过抑制神经生长因子（NGF）诱导的神经突生长所测量的。然而，卟啉前体δ-氨基乙酰丙酸（δ-ALA）在30小时的时间段内在高达毫摩尔浓度下是无毒的。两种合成卟啉，四苯基卟啉磺酸盐（TPPS）和血卟啉衍生物（HPD），也已被证明是有毒的体外。在卟啉对完整神经系统影响的研究中，中枢和外周神经系统来源的神经元和神经胶质细胞的分离群体的培养和共培养可能被证明是有利的。这种体外检测系统可以补充体内的范例，并可能是有用的快速定量筛选放射增敏剂，包括合成卟啉和其他化疗药物的神经毒性。
Using a dissociated primary sensory neuron culture system, it is observed that some naturally occurring porphyrins produce dose-dependent neurotoxicity as measured by neuron death and by inhibition of the neurite outgrowth induced by Nerve Growth Factor (NGF). However, the porphyrin precursor delta-aminolevulinic acid (delta-ALA) is not toxic up to millimolar concentrations within a 30-h time period. Two synthetic porphyrins, tetraphenylporphine sulfonate (TPPS) and hematoporphyrin derivative (HPD), have also been shown to be toxic in vitro. Cultures and cocultures of isolated populations of neurons and glia of central and peripheral nervous system origin may prove advantageous in the study of porphyrin influences on the intact nervous system. This in vitro assay system can complement in vivo paradigms and may be useful for rapid quantitative screening for neurotoxicity of radiation sensitizers including the synthetic porphyrins and other chemotherapeutic agents.