TRANSLATIONAL INHIBITION BY CYTOMEGALOVIRUS TRANSCRIPT LEADERS
TRANSLATIONAL INHIBITION BY CYTOMEGALOVIRUS TRANSCRIPT LEADERS
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DOI：
10.1016/0042-6822(90)90531-u
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发表时间：
1990-08-01
期刊：
影响因子：
3.7
通讯作者：
GEBALLE, AP
中科院分区：
文献类型：
--
作者：
BIEGALKE, BJ;GEBALLE, AP
人巨细胞病毒基因表达的调控依赖于转录和转录后调控。先前的研究表明，在β的5“前导序列中包含的两个AUG密码子中的任何一个都可以被编码。基因（2.7 β）转录抑制下游阅读框的翻译。我们研究了来自巨细胞病毒DNA聚合酶和pp150基因的5“前导序列的调节作用，每个前导序列也包含上游AUG密码子。令人惊讶的是，这两个前导序列不影响下游开放阅读弗罗姆的表达。进行了详细的分析，以检查pp150前导序列中AUG密码子的作用。这些上游AUG密码子允许有效的下游翻译，尽管真核翻译的扫描模型的预测。2.7.beta的进一步研究。前导序列揭示了上游AUG密码子虽然是必需的，但不足以抑制下游翻译。这些结果表明，CMV转录前导序列的翻译抑制需要一个AUG密码子和额外的前导序列。
The regulation of human cytomegalovirus gene expression depends on both transcriptional and post-transcriptional controls. Previous studies revealed that either of two AUG codons contained in the 5'' leader of a .beta. gene (2.7.beta.) transcript inhibited translation of a downstream reading frame. We investigated the regulatory effects of 5'' leader sequences from the cytomegalovirus DNA polymerase and pp150 genes, each of which also contains upstream AUG codons. Surprisingly, these two leaders did not affect expression of the downstream open reading frome. Detailed analyses were carried out to examine the role of the AUG codons within the pp150 leader. These upstream AUG codons allowed efficient downstream translation, despite the predictions of the scanning model of eukaryotic translation. Further studies of the 2.7.beta. leader revealed that an upstream AUG codon, although necessary, was not sufficient to inhibit downstream translation. These results reveal that translational inhibition by CMV transcript leaders requires an AUG codon and additional leader sequences.