Oxidative production of monomethylated selenium from the major urinary selenometabolite, selenosugar
Oxidative production of monomethylated selenium from the major urinary selenometabolite, selenosugar
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DOI：
10.1039/b306710f
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发表时间：
2003-01-01
影响因子：
3.4
通讯作者：
Suzuki, KT
中科院分区：
文献类型：
--
作者：
Ogra, Y;Hatano, T;Suzuki, KT
采用高效液相色谱-电感耦合氩等离子体质谱法检测大鼠尿液中除主要的硒代谢物1 - β -甲基硒- n -乙酰- d -半乳糖胺（硒糖）外，还有几种次要的硒代谢物。其中一种是在部分纯化的尿液样本中，由硒糖在有氧条件下产生，并通过H2O2氧化转化。经鉴定为单甲基亚硒酸。因此，这种尿硒化合物不应被视为一种自然产生的代谢物，而应被视为一种硒糖产物。此外，在相同的尿液样本中检测到额外的硒代谢物。虽然这种硒代谢物尚未被鉴定，但由于其通过氧化转化为单甲基亚硒酸，似乎含有单甲基亚硒基。因此，尿液样本必须小心处理，以免被空气氧化，以防止转化为人工硒化合物。
In addition to the major urinary selenometabolite, 1beta-methylseleno-N-acetyl-D-galactosamine (selenosugar), several minor selenometabolites were also detected in rat urine by high performance liquid chromatography inductively coupled argon plasma mass spectrometry. One of them, in a partially purified urine sample, was generated from selenosugar under aerobic conditions and also transformed through oxidation by H2O2. It was identified as monomethylseleninic acid. Therefore, this urinary selenocompound should not be regarded as a naturally occurring metabolite but as a selenosugar artifact. Moreover, an additional selenometabolite was detected in the same urine sample. Although this selenometabolite has not been identified yet, it seems to contain monomethylselenyl group(s) because of its transformation to monomethylseleninic acid through oxidation. Thus, urine samples must be handled with care so as not to allow oxidation by air, to prevent transformation to artificial selenocompounds.