Differential expression profiling of microRNAs and their potential involvement in renal cell carcinoma pathogenesis
Differential expression profiling of microRNAs and their potential involvement in renal cell carcinoma pathogenesis
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DOI：
10.1016/j.clinbiochem.2009.07.020
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发表时间：
2010-01-01
影响因子：
2.8
通讯作者：
Yousef, George M.
中科院分区：
文献类型：
--
作者：
Chow, Tsz-Fung F.;Youssef, Youssef M.;Yousef, George M.
目的：设计和方法：我们进行了一个miRNA微阵列分析，比较肾透明细胞癌组织及其正常对照组织之间的miRNA表达水平。通过定量RT-PCR分析来验证前失调的miRNA。结果：共检测到33种在肾细胞癌中表达异常的miRNAs，其中21种表达上调，其中许多miRNAs在其他恶性肿瘤中表达异常，可能与肿瘤的发生发展有关。这些miRNAs与已报道的染色体畸变位点具有显著相关性。我们还利用目标预测算法来识别基因靶标。初步分析表明，这些靶点可能直接参与了肾细胞癌的发病机制。结论：我们发现了在肾细胞癌中表达异常的miRNAs，生物信息学分析表明这些miRNAs可能参与了肾细胞癌的发病机制，并有可能成为肾细胞癌的生物标志物。(c)2009年加拿大临床化学家协会。爱思唯尔公司出版All rights reserved.
Objective: We seek to identify the differentially expressed miRNAs in the clear cell subtype (ccRCC) of kidney cancer.Design and methods: We performed a miRNA microarray analysis to compare the miRNA expression levels between ccRCC tissues and their normal Counterpart. The top dysregulated miRNAs were validated by quantitative RT-PCR analysis. Bioinformatics analysis was also performed.Results: A total of 33 dysregulated miRNAs were identified in ccRCC, including 21 upregulated miRNAs and many of these miRNAs have been reported to be dysregulated in other malignancies and have a potential role in cancer pathogenesis. The miRNAs showed a significant correlation with reported chromosomal aberration sites. We also utilized target prediction algorithms to identify gene targets. Preliminary analyses showed these targets can be directly involved in RCC pathogenesis.Conclusion: We identified miRNAs that are dysregulated in ccRCC and bioinformatics analysis suggests that these miRNAs may be involved in cancer pathogenesis and have the potential to be biomarkers. (c) 2009 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.