Assessment of bacterial communities and characterization of lead-resistant bacteria in the rhizosphere soils of metal-tolerant Chenopodium ambrosioides grown on lead-zinc mine tailings.
Assessment of bacterial communities and characterization of lead-resistant bacteria in the rhizosphere soils of metal-tolerant Chenopodium ambrosioides grown on lead-zinc mine tailings.
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DOI：
10.1016/j.chemosphere.2012.02.036
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发表时间：
2012-06
期刊：
影响因子：
8.8
通讯作者：
Wen-hui Zhang;Zhi Huang;Linyan He;X. Sheng
中科院分区：
文献类型：
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作者：
Wen-hui Zhang;Zhi Huang;Linyan He;X. Sheng
采用独立栽培和栽培技术，对重、中度铅锌尾矿污染土壤以及邻近低度金属污染土壤中生长的耐金属积累性藜的根际土壤中的细菌群落进行了表征。在高、中、低污染土壤克隆文库中分别检测到与数据库中最接近匹配的69、73和83个细菌操作分类单元（OTU），相似度为84.8-100%。这些 OTU 的香农多样性指数值在 4.06-4.30 范围内。分别有9、10和14个针对高、中和低金属污染土壤克隆文库的细菌属。系统发育分析表明，耐铅菌株属于8个属。分离株中以假单胞菌和节杆菌为主。大多数分离株（82-86%）产生吲哚乙酸和铁载体。来自高度金属污染土壤的菌株比来自中度和轻度金属污染土壤的菌株更多地产生1-氨基环丙烷-1-羧酸脱氨酶。在涉及在含有 200mgkg−1 Pb 的石英砂中生长的双低油菜籽的实验中，用分离的 Jaamilae 类芽孢杆菌 HTb8 和假单胞菌接种。与未接种的对照相比，GTa5 显着增加了地上组织干重（19% 至 36%）和 Pb 吸收（30% 至 40%）。这些结果表明，C. ambrosioides在其根际土壤中含有不同的抗金属细菌群落，表达植物促生长性状的分离株可用于提高铅污染环境的植物提取效率。
Bacterial communities in the rhizosphere soils of metal tolerant and accumulating Chenopodium ambrosioides grown in highly and moderately lead–zinc mine tailings contaminated-soils as well as the adjacent soils with low metal contamination were characterized by using cultivation-independent and cultivation techniques. A total of 69, 73, and 83 bacterial operational taxonomic units (OTUs) having 84.8–100% similarity with the closest match in the database were detected among high, moderate, and low-contamination soil clone libraries, respectively. These OTUs had a Shannon diversity index value in the range of 4.06–4.30. There were 9, 10, and 14 bacterial genera specific to high, moderate, and low metal-contaminated soil clone libraries, respectively. Phylogenetic analysis showed that the Pb-resistant isolates belonged to 8 genera. Pseudomonas and Arthrobacter were predominant among the isolates. Most of the isolates (82–86%) produced indole acetic acid and siderophores. More strains from the highly metal-contaminated soil produced 1-aminocyclopropane-1-carboxylate deaminase than the strains from the moderately and lowly metal-contaminated soils. In experiments involving canola grown in quartz sand containing 200mgkg−1of Pb, inoculation with the isolated Paenibacillusjamilae HTb8 and Pseudomonas sp. GTa5 was found to significantly increase the above-ground tissues dry weight (ranging from 19% to 36%) and Pb uptake (ranging from 30% to 40%) compared to the uninoculated control. These results show that C. ambrosioides harbor different metal-resistant bacterial communities in their rhizosphere soils and the isolates expressing plant growth promoting traits may be exploited for improving the phytoextraction efficiency of Pb-polluted environment.