The yeast Pan2 protein is required for poly(A)-binding protein-stimulated poly(A)-nuclease activity
The yeast Pan2 protein is required for poly(A)-binding protein-stimulated poly(A)-nuclease activity
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DOI：
10.1074/jbc.271.1.432
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发表时间：
1996-01-05
影响因子：
4.8
通讯作者：
Sachs, AB
中科院分区：
文献类型：
--
作者：
Boeck, R;Tarun, S;Sachs, AB
在酵母酿酒酵母中，mRNA poly（A）尾的去除被poly（A）结合蛋白（Pab 1 p）刺激。对Pab 1 p刺激的poly（A）核糖核酸酶（PAN）的大规模纯化鉴定出一个76-kDa和两个135-Da多肽作为候选酶亚基。针对Pan 1 p蛋白的抗体，Pan 1 p蛋白是制备中的次要135-kDa蛋白，主要的135-kDa蛋白质Pan 2 p的蛋白质序列揭示了一种新的蛋白质，该蛋白质也在先前报道的PAN纯化中发现（Sachs，A. B.，和Deardorff，J. A，（1992）Cell 70，961-973）。非必需PAN 2基因的缺失导致体内mRNA poly（A）尾的平均长度增加，并且在粗提物中Pab 1 p刺激的PAN活性丧失。这些数据证实PAN活性需要Pan 2 p而不是Pan 1 p，并且它们表明除了Pab 1 p刺激的PAN之外的核糖核酸酶能够缩短体内poly（A）尾。
The removal of the mRNA poly(A) tail in the yeast Saccharomyces cerevisiae is stimulated by the poly(A)binding protein (Pab1p), A large scale purification of the Pab1p-stimulated poly(A) ribonuclease (PAN) identifies a 76-kDa and two 135-Da polypeptides as candidate enzyme subunits, Antibodies against the Pan1p protein, which is the minor 135-kDa protein in the preparation, can immunodeplete Pan1p but not PAN activity, The protein sequence of the major 135-kDa protein, Pan2p, reveals a novel protein that was also found in the previously reported PAN purification (Sachs, A. B., and Deardorff, J. A, (1992) Cell 70, 961-973). Deletion of the non-essential PAN2 gene results in an increase of the average length of mRNA poly(A) tails in vivo, and a loss of Pab1p-stimulated PAN activity in crude extracts, These data confirm that Pan2p and not Pan1p is required for PAN activity, and they suggest that ribonucleases other than the Pab1p-stimulated PAN are capable of shortening poly(A) tails in vivo.