Regulation of pulsatile gonadotropin secretion by estrogen, inhibin, and follistatin (activin-binding protein) in ovariectomized rats.
Regulation of pulsatile gonadotropin secretion by estrogen, inhibin, and follistatin (activin-binding protein) in ovariectomized rats.
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卵巢切除大鼠中雌激素、抑制素和卵泡抑素（激活素结合蛋白）对脉动促性腺激素分泌的调节。
DOI：
10.1095/biolreprod46.5.898
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发表时间：
1992
影响因子：
3.6
通讯作者：
Ling,N
中科院分区：
文献类型：
--
作者：
Depaolo,LV;Shimonaka,M;Ling,N
接下来的研究考察了单独或联合给予雌激素和多肽对搏动性促性腺激素分泌的影响。卵巢摘除后1周，皮下注射。注射油或不同剂量(0.5、5、20微克)的苯甲酸雌二醇(EB)，1天后静脉注射。给予60微克纯化的猪卵泡抑素、10微克重组抑制素或适当的载体。注射非类固醇4小时后，每隔10分钟采血2小时，观察其对搏动性激素释放的影响。单独给药EB剂量依赖地抑制平均和谷值(两个脉冲之间的最低点)FSH水平和所有参数的脉动性黄体生成素释放。卵泡抑素和抑制素在所用剂量下对平均FSH水平的抑制程度相当(40%)。卵泡刺激素可抑制FSH脉冲幅度，但抑制素不能抑制FSH脉冲幅度，但这两种多肽均不能单独影响FSH脉冲频率或任何参数。在所有EB剂量下，卵泡抑素和EB对平均FSH水平的影响是相加的，而抑制素和EB只在中等剂量时才是相加的。卵泡抑素联合最低EB剂量显著抑制平均促黄体生成素水平。这些研究首次证明，雌激素与非甾体激素、卵泡抑素和抑制素联合使用比单独使用任何一种药物都能更有效地抑制FSH的释放，从而表明类固醇和非甾体激素可能在大鼠FSH分泌的生理调节中起着重要作用。这些化合物对FSH分泌的相加作用可能成为探索新的避孕措施的基础。
The following study was conducted to examine the effects of estrogen and polypeptides, given either alone or in combination, on pulsatile gonadotropin secretion. One week after ovariectomy, rats received s.c. injections of oil or various doses (0.5, 5, 20 µg) of estradiol benzoate (EB) followed 1 day later by i.v. administration of 60 µg purified porcine follistatin, 10 µg recombinant inhibin, or the appropriate vehicle. Four hours after injection of the nonsteroids, blood was collected at 10-min intervals for 2 h, and the effects on pulsatile hormone release were assessed. Administration of EB alone dose-dependently suppressed mean and trough (lowest point between two pulses) FSH levels and all parameters of pulsatile LH release. Both follistatin and inhibin at the doses employed suppressed mean FSH levels to an equivalent extent (40%). Follistatin, but not inhibin, suppressed FSH pulse amplitude, while neither polypeptide alone influenced FSH pulse frequency or any parameter of pulsatile LH release. The effects of follistatin and EB on mean FSH levels were additive at all EB doses, whereas the effects of inhibin and EB were additive only at the middle EB dose. Follistatin in combination with the lowest EB dose significantly suppressed mean LH levels. These studies are the first to demonstrate that combined treatment with estrogen and the nonsteroids follistatin and inhibin is more efficacious in suppressing FSH release than treatment with either agent alone, thereby indicating that both steroids and nonsteroids are probably important in the physiological regulation of FSH secretion in rats. The additive effects of these compounds on FSH secretion could form the basis for exploring novel contraceptive interventions.