Biochemical and hematologic values for 18 clinically healthy radiated tortoises (Geochelone radiata) on St Catherines Island, Georgia.
Biochemical and hematologic values for 18 clinically healthy radiated tortoises (Geochelone radiata) on St Catherines Island, Georgia.
复制标题
佐治亚州圣凯瑟琳斯岛 18 只临床健康的放射陆龟 (Geochelone radiata) 的生化和血液学值。
DOI：
10.1111/j.1939-165x.2006.tb00139.x
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发表时间：
2006
影响因子：
1.2
通讯作者：
Cray,Carolyn
中科院分区：
文献类型：
--
作者：
Zaias,Julia;Norton,Terry;Fickel,Angela;Spratt,Jeff;Altman,NormanH;Cray,Carolyn
辐射龟（Geochelone radiata）是马达加斯加岛南部的一种极度濒危物种。人工繁殖计划产生的数据，繁殖行为和生态学的G辐射;本研究的目的是评估性别和季节对圈养辐射陆龟血液学和生化指标的影响（10只雄性，8只雌性），圈养在格鲁吉亚圣凯瑟琳岛的野生动物生存中心，作为常规健康评估的一部分。使用标准方法进行常规血液学和血浆生化分析和电泳。从男性和女性龟的数据进行了比较，在季节内和季节之间使用2-way ANOVA.ResultsRBC和HCT值显着高于夏季比冬季，男性高于女性。总蛋白浓度在雄性和雌性之间没有显著差异;然而，雌性陆龟在冬季和夏季的α1-和β-球蛋白浓度显著高于雄性陆龟。与雌龟相比，雄龟在冬季钠、尿酸浓度和LDH活性显著升高，在夏季尿素浓度和LDH、CK活性显著升高。雌性陆龟有显着较高的甘油三酯和磷浓度在冬季，更高的磷，胆固醇，甘油三酯浓度在夏季，与男性相比。ConclusionSex和季节差异辐射陆龟的血液学和生化值可能反映卵黄发生和产卵的女性，并改变水合状态和活动在夏季。本研究中来自陆龟的数据将有助于该物种的季节性健康评估。
BackgroundThe radiated tortoise (Geochelone radiata) is a critically endangered species in its native land, the southern portion of the island of Madagascar. Captive breeding programs have generated data on the breeding behavior and ecology ofG radiata; however, hematologic and biochemical data also are critically important in managing populations.ObjectiveThe purpose of this study was to evaluate sex and seasonal effects on hematologic and biochemical data from captive radiated tortoises.MethodsWhole blood was collected in January and August 2001 from 18 radiated tortoises (10 male, 8 female) housed at the Wildlife Survival Center on St Catherines Island, Georgia, as part of a routine health assessment. Routine hematologic and plasma biochemical analyses and electrophoresis were done using standard methods. Data from male and female tortoises were compared within and between seasons using 2‐way ANOVA.ResultsRBC and HCT values were significantly higher in summer than in winter and were higher in males than in females. Total protein concentration did not differ significantly between males and females; however, female tortoises had significantly higher concentrations of α1‐ and β‐globulins in winter and summer compared to males. Male tortoises had significantly higher sodium and uric acid concentrations and LDH activity during winter, and higher urea concentration and LDH and CK activities in summer, compared with females. Female tortoises had significantly higher triglyceride and phosphorus concentrations in winter, and higher phosphorus, cholesterol, and triglyceride concentrations in summer, compared with males.ConclusionSex and seasonal differences in hematologic and biochemical values for radiated tortoises likely reflect vitellogenesis and egg production in females, and altered hydration status and activity in summer. Data from the tortoises in this study will be useful for the seasonal health assessment of this species.