The role of a groundwater bacterial community in the degradation of the herbicide terbuthylazine
The role of a groundwater bacterial community in the degradation of the herbicide terbuthylazine
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DOI：
10.1111/j.1574-6941.2009.00787.x
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发表时间：
2010-01-01
影响因子：
4.2
通讯作者：
Gibello, Alicia
中科院分区：
文献类型：
--
作者：
Barra Caracciolo, Anna;Fajardo, Carmen;Gibello, Alicia
研究了受均三嗪污染的含水层中的细菌群落。用浓度为 100 μg L-1 的特丁嗪处理地下水微观世界，并评估除草剂的降解情况。分析了细菌群落结构（丰度和系统发育组成）和功能（碳产生和细胞活力）。细菌群落能够降解特丁嗪；特别是，β变形菌参与除草剂生物转化。通过 16S rRNA 基因的 PCR 扩增对一些细菌分离株进行鉴定，发现存在两种能够降解除草剂的 Betaproteobacteria：Advenella incenata 和 Janthinobacteria lividum。对编码均三嗪降解酶的基因进行 PCR 检测表明，A. incenata 中存在 atzA 和 atzB 基因，而 J. lividum 中存在 atzB 和 atzC 基因。这些菌株的atz基因的PCR片段的核苷酸序列与假单胞菌属的同源基因100%相同。应变ADP。总之，结果显示了使用自然衰减策略处理受特丁嗪污染的含水层的潜力。分离出的两种细菌可以促进有效生物修复方案的实施，特别是在地下水因意外泄漏而发现大量除草剂的情况下。
A bacterial community in an aquifer contaminated by s-triazines was studied. Groundwater microcosms were treated with terbuthylazine at a concentration of 100 mu g L-1 and degradation of the herbicide was assessed. The bacterial community structure (abundance and phylogenetic composition) and function (carbon production and cell viability) were analysed. The bacterial community was able to degrade the terbuthylazine; in particular, Betaproteobacteria were involved in the herbicide biotransformation. Identification of some bacterial isolates by PCR amplification of the 16S rRNA gene revealed the presence of two Betaproteobacteria species able to degrade the herbicide: Advenella incenata and Janthinobacterium lividum. PCR detection of the genes encoding s-triazine-degrading enzymes indicated the presence of the atzA and atzB genes in A. incenata and the atzB and atzC genes in J. lividum. The nucleotide sequences of the PCR fragments of the atz genes from these strains were 100% identical to the homologous genes of the Pseudomonas sp. strain ADP. In conclusion, the results show the potential for the use of a natural attenuation strategy in the treatment of aquifers polluted with the terbuthylazine. The two bacteria isolated could facilitate the implementation of effective bioremediation protocols, especially in the case of the significant amounts of herbicide that can be found in groundwater as a result of accidental spills.