Intracerebroventricular administration of sulphated cholecystokinin octapeptide induces anxiety-like behaviour in goldfish
Intracerebroventricular administration of sulphated cholecystokinin octapeptide induces anxiety-like behaviour in goldfish
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脑室内注射硫酸化胆囊收缩素八肽可诱导金鱼的焦虑样行为
DOI：
10.1111/jne.12667
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发表时间：
2019
影响因子：
3.2
通讯作者：
Matsuda Kouhei
中科院分区：
文献类型：
--
作者：
Sachuriga;Iinuma Naoto;Shibata Haruki;Yoshida Daisuke;Konno Norifumi;Nakamachi Tomoya;Matsuda Kouhei
硫酸胆囊收缩素八肽（CCK-8 s）是脊椎动物的一种促摄食神经肽，参与摄食调节。在啮齿类动物中，CCK-8的i. c. v.给药已被证明不仅影响进食行为，而且影响精神活动。然而，仍然没有关于CCK-8对金鱼的心理生理影响的信息。因此，在本研究中，我们研究了合成金鱼（gf）CCK-8 s对该物种精神活动的影响。以0.1、0.5和2.5 pmol g-1体重（BW）脑室注射gfCCK-8不会影响游泳距离（自发活动）。因为金鱼更喜欢水箱的下部而不是上部，所以我们将其用作偏好测试（上/下测试）来评估焦虑样行为。以2.5 pmol g-1BW脑室注射gfCCK-8 s缩短了在上部区域花费的时间。在5和10 pmol g-1BW下，gfCCK-8的作用模拟FG-7142（中枢型苯二氮卓受体反向激动剂，一种致焦虑剂）的作用。gfCCK-8 s的致焦虑样作用可通过CCK受体拮抗剂丙谷胺（50 pmol g-1BW）治疗消除。我们还研究了金鱼脑中CCK/胃泌素样免疫反应性的定位。在焦虑相关区域（缰核和脚间核）观察到CCK/胃泌素样免疫反应。这些数据表明，gfCCK-8 s有效地影响金鱼的精神活动，并通过CCK受体信号通路发挥致焦虑样作用。
Sulphated cholecystokinin octapeptide (CCK‐8s) is involved in feeding regulation as an anorexigenic neuropeptide in vertebrates. In rodents, i.c.v. administration of CCK‐8s has been shown to affect not only feeding behaviour, but also psychomotor activity. However, there is still no information available concerning the psychophysiological effects of CCK‐8s in goldfish. Therefore, in the present study, we examined the effect of synthetic goldfish (gf) CCK‐8s on psychomotor activity in this species. Intracerebroventricular administration of gfCCK‐8s at 0.1, 0.5 and 2.5 pmol g‐1body weight (BW) did not affect swimming distance (locomotor activity). Because goldfish prefer the lower to the upper area of a tank, we used this as a preference test (upper/lower test) to assess anxiety‐like behaviour. Intracerebroventricular administration of gfCCK‐8s at 2.5 pmol g‐1BW shortened the time spent in the upper area. The action of gfCCK‐8s mimicked that of FG‐7142 (the central‐type benzodiazepine receptor inverse agonist, an anxiogenic agent) at 5 and 10 pmol g‐1BW. The anxiogenic‐like effect of gfCCK‐8s was abolished by treatment with the CCK receptor antagonist proglumide at 50 pmol g‐1BW. We also investigated the localisation of CCK/gastrin‐like immunoreactivity in the goldfish brain. CCK/gastrin‐like immunoreactivity was observed in the anxiety‐related regions (the nucleus habenularis and the interpeduncular nucleus). These data indicate that gfCCK‐8s potently affects psychomotor activity in goldfish, and exerts an anxiogenic‐like effect via the CCK receptor‐signalling pathway.