Protective effect of fluvastatin on influenza virus infection
Protective effect of fluvastatin on influenza virus infection
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氟伐他汀对流感病毒感染的保护作用
DOI：
10.3892/mmr.2014.2076
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发表时间：
2014-06-01
影响因子：
3.4
通讯作者：
Lu, Jiahai
中科院分区：
文献类型：
--
作者：
Peng, Jing;Zhang, Dingmei;Lu, Jiahai
他汀类药物是3-羟基-3-甲基戊二酰辅酶A还原酶抑制剂，具有多效性。有人建议在下一次流感大流行期间，他汀类药物可能是一种潜在的治疗方法。在之前的一项研究中，我们发现他汀类和咖啡因的组合可以保护BALB/c小鼠免受甲型流感、血凝素5亚型和神经氨酸酶1(H5N1)、H3N2和H1N1的感染。然而，他汀类药物单独对流感病毒感染的影响尚不清楚。本研究探讨氟伐他汀在体外是否具有抑制甲型流感病毒复制的作用。结果表明，氟伐他汀对病毒RNA和蛋白质的合成有影响。当氟伐他汀与病毒同时给药时，病毒产量显著减少；然而，当在病毒吸附后加入氟伐他汀时，观察到更大的抑制作用。然而，选择性指数[SI；50%细胞毒性浓度(CC50)/50%抑制浓度(IC50)]仅为21。进一步证明，氟伐他汀通过减少肿瘤坏死因子-α、白介素8和干扰素-γ的产生，保护宿主细胞免受流感诱导的炎症。综上所述，氟伐他汀对流感病毒感染有轻微的抑制作用，其机制可能与抗炎作用有关。
Statins are 3-hydroxy-3-methylglutaryl coenzyme A reductase inhibitors and have pleiotropic effects. It has been suggested that statins may be a potential treatment during the next influenza pandemic. In a previous study we found that a statin/caffeine combination protects BALB/c mice against Influenza A, subtypes haemagglutinin type 5 and neuraminidase type 1 (H5N1), H3N2 and H1N1 infection. The effect of statins alone on influenza virus infection, however, is not known. In this study, it was investigated whether fluvastatin is capable of inhibiting influenza A virus replication in vitro. The results demonstrated that the synthesis of viral RNA and protein was affected by fluvastatin treatment. Virus production was markedly reduced when fluvastatin was administered simultaneously with the virus; however, a greater inhibition was observed when fluvastatin was added following viral adsorption. The selectivity index [SI; 50% cytotoxic concentration (CC50)/50% inhibition concentration (IC50)], however, was only 21. It was further demonstrated that fluvastatin protects host cells against influenza-induced inflammation by reducing the production of tumour necrosis factor-alpha, interleukin 8 and interferon gamma. In conclusion, the results demonstrated that fluvastatin exerted a minor inhibitory effect on influenza virus infection, which involved anti-inflammatory activities.