Natural variation and drought-induced differences in metabolite profiles of red oak-leaf and Romaine lettuce play a role in modulating the interaction with Salmonella enterica
Natural variation and drought-induced differences in metabolite profiles of red oak-leaf and Romaine lettuce play a role in modulating the interaction with Salmonella enterica
复制标题
红橡叶和长叶生菜的自然变化和干旱引起的代谢物差异在调节与肠沙门氏菌的相互作用中发挥着作用
DOI：
10.1016/j.ijfoodmicro.2022.109998
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发表时间：
2023
影响因子：
5.4
通讯作者：
Micallef, Shirley A.
中科院分区：
文献类型：
--
作者：
Liu, Xingchen;Li, Yue;Micallef, Shirley A.
农产品表面的营养物质对肠道病原体的成功定植至关重要。在这项研究中，我们研究了长叶莴苣“Parris Island Cos”和红橡树叶莴苣“Mascara”在常规和限制浇水条件下代谢物谱的自然变化。我们还研究了植物干旱胁迫对沙门氏菌-生菜关联的影响。在定期浇水的长叶莴苣上，沙门氏菌和鼠伤寒杆菌的含量比红橡树叶莴苣高。干旱处理对生菜附生沙门氏菌的影响；新港鼠和鼠伤寒鼠受到不同程度的影响。受干旱影响的红橡树叶莴苣植株的两种血清型的对数降低率均高于对照。鼠伤寒杆菌对水分亏缺处理的长叶莴苣无显著影响（p< 0.05）。为了评估沙门氏菌与叶片表面代谢物的相互作用，从两个品种收集的叶片洗涤液进行接种，发现能够支持。从长叶莴苣中提取的沙门氏菌含量较高（p< 0.05）。s的滞后阶段。限水红橡树叶生菜水洗后的新港期较常规浇水的对照组延长（p< 0.05）。利用电喷雾电离飞行时间质谱（ESI-TOF-MS）分析非靶向植物代谢物，揭示了长叶莴苣和红橡树叶莴苣在叶组织和叶洗方面的自然差异。在干旱胁迫下，两种生菜的代谢谱都发生了变化，但干旱处理后，红橡树叶的代谢谱出现了更多的独特峰。两种品种之间的差异部分归因于红橡树叶莴苣中黄酮类化合物和花青素的天然含量高于长叶莴苣。此外，红橡树叶对干旱的反应主要是诱导脯氨酸、酚类物质、黄酮类物质和花青素的积累，而长叶莴苣则不是。因此，干旱胁迫增强了红橡树叶莴苣的功能性食品特性。生菜叶洗液中沙门氏菌的生长动态表明，自然变化和干旱诱导的生菜代谢物谱的变化可以部分解释不同生菜类型对沙门氏菌的不同易感性，尽管介导这种影响的初级或次级代谢物尚不清楚。应研究调节轻度水分胁迫的方法，以降低适宜生菜品种的沙门氏菌污染风险，同时提高生菜的健康品质。
Nutrients on produce surfaces are vital for successful enteric pathogen colonisation. In this study, we investigated natural variation in metabolite profiles of Romaine ‘Parris Island Cos’ and red oak-leaf lettuce ‘Mascara’ under regular and restricted watering conditions. We also investigated the impact of plant drought stress on theSalmonella– lettuce association.SalmonellaNewport and Typhimurium were able to persist at higher levels on regularly watered Romaine than red oak-leaf lettuce. Drought treatment to lettuce impaired epiphyticSalmonellaassociation, withS.Newport and Typhimurium being differentially affected. A higher log reduction of both serotypes was measured on drought-subjected red oak-leaf lettuce plants than controls, butS.Typhimurium was unaffected on water deficit-treated Romaine lettuce(p< 0.05).To assessSalmonellainteraction with leaf surface metabolites, leaf washes collected from both cultivars were inoculated and found to be able to supportS.Newport growth, with higher levels ofSalmonellaretrieved from Romaine washes (p< 0.05). The lag phase ofS.Newport in washes from water restricted red oak-leaf lettuce was prolonged in relation to regularly-watered controls (p< 0.05). Untargeted plant metabolite profiling using electrospray ionization time-of-flight mass spectrometry (ESI-TOF-MS) revealed natural variation between Romaine and red oak-leaf lettuce profiles for leaf tissue and leaf washes. Metabolite profile shifts were detected in both lettuce types in response to drought stress, but more unique peaks were detected in red oak-leaf than Romaine lettuce after drought treatment. Variation between the two cultivars was in part attributed to naturally higher levels of flavonoids and anthocyanins in red oak-leaf lettuce compared to Romaine. Moreover, red oak-leaf, but not Romaine lettuce, responded to drought by inducing the accumulation of proline, phenolics, flavonoids and anthocyanins. Drought stress, therefore, enhanced the functional food properties of red oak-leaf lettuce.Salmonellagrowth dynamics in lettuce leaf washes suggested that natural variation and drought-induced changes in metabolite profiles in lettuce could partly explain the differential susceptibility of various lettuce types toSalmonella, although the primary or secondary metabolites mediating this effect remain unknown. Regulated mild water stress should be investigated as an approach to lowerSalmonellacontamination risk in suitable lettuce cultivars, while simultaneously boosting the health beneficial quality of lettuce.