Ubiquitin ligase gene neurl3 plays a role in spermatogenesis of half-smooth tongue sole (Cynoglossus semilaevis) by regulating testis protein ubiquitination
Ubiquitin ligase gene neurl3 plays a role in spermatogenesis of half-smooth tongue sole (Cynoglossus semilaevis) by regulating testis protein ubiquitination
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泛素连接酶基因 neurl3 通过调节睾丸蛋白泛素化在半滑舌鳎 (Cynoglossus semilaevis) 精子发生中发挥作用
DOI：
10.1016/j.gene.2016.07.062
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发表时间：
2016-10-30
期刊：
影响因子：
3.5
通讯作者：
Chen, Songlin
中科院分区：
文献类型：
--
作者：
Xu, Wenteng;Li, Hailong;Chen, Songlin
E3泛素连接酶是一个在精子发生中发挥多种作用的大基因家族。在这项研究中，神经化的E3泛素蛋白连接酶3(Nerl3)的功能特性揭示了它参与精子发生的可能性。首先，我们发现在睾丸生殖细胞中，Neurl3的转录存在男性偏向，并且其翻译占主导地位。通过RNA干扰抑制Neurl3基因，导致精子发生相关基因转录增加。这些结果证实了之前的研究，表明了神经元3在精子发生中的作用。此外，神经原3转录水平与睾丸蛋白泛素化密切相关。基于这些发现，我们推测，神经元3以一种剂量依赖的方式调节睾丸蛋白的泛素化，并影响精子的发生。(C)2016爱思唯尔B.V.保留所有权利。
E3 ubiquitin ligases are a large gene family that plays a diversity of roles in spermatogenesis. In this study, the functional characterization of a neuralized E3 ubiquitin protein ligase 3 (neurl3) revealed its potential participation in spermatogenesis. Firstly, we found that neurl3 exhibited male-biased transcription and that its translation was predominant in testis germ cells. The knockdown of neurl3 by RNA interference caused increased transcription of spermatogenesis-related genes. These results corroborate previous studies indicating a role for neurl3 in spermatogenesis. Moreover, the levels of neurl3 transcription and testis protein ubiquitination were closely correlated. Based on these findings, we speculate that neurl3 modulates testis protein ubiquitination in a dosage-dependent manner and that this influences spermatogenesis. (C) 2016 Elsevier B.V. All rights reserved.