Stable earthworm serine proteases: Application of the protease function and usefulness of the earthworm autolysate
Stable earthworm serine proteases: Application of the protease function and usefulness of the earthworm autolysate
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DOI：
10.1016/s1389-1723(00)80106-1
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发表时间：
2000-08-01
影响因子：
2.8
通讯作者：
Ishihara, K
中科院分区：
文献类型：
--
作者：
Nakajima, N;Sugimoto, M;Ishihara, K
红地龙[Lumbricus rubellus [Nakajima，N.例如，Biosci. Biotechnol.生物化学。，57，1726-1730（1993），60，293-300（1996）和63，2031-2033（1999）]进一步表征以利用它们的催化功能。这些酶在室温下在溶液中长时间稳定，并且对有机溶剂，甚至甲苯和正己烷具有很强的耐受性。丝氨酸蛋白酶可以作用于各种蛋白质底物，如弹性蛋白和血红蛋白以及纤维蛋白，并且还催化酯如乙酸乙酯和生物塑料聚[（R）-3-羟基丁酸酯]膜的水解。在不发生微生物降解的情况下，该酶能产生具有抗氧化能力和蛋白酶活性的酱油自溶物，其成分与酱料中的成分相似。该菌自溶物的提取物可用作微生物培养培养基中的蛋白胨替代物。
The fibrinolytic enzymes from Lumbricus rubellus [Nakajima, N. et al., Biosci. Biotechnol. Biochem., 57, 1726-1730 (1993), 60, 293-300 (1996), and 63, 2031-2033 (1999)] were further characterized to exploit their catalytic functions. These enzymes are stable in solution for long periods at room temperature and strongly resistant to organic solvents, even toluene and n-hexane. The serine proteases can act on various protein substrates such as elastin and hemoglobin as well as fibrin, and also catalyzed the hydrolysis of esters such as ethyl acetate and a bioplastic, poly[(R)-3-hydroxybutyrate] film. The enzymes, In the absence of microbial degradation, contributed to the production of the earthworm autolysate possessing antioxidant ability and protease activity, whose components were similar to those of soy sauce. The extract of the earthworm autolysate could be used as a peptone substitute in media for the cultivation of microorganisms.