EXPRESSION OF THE GENE FOR RIBOSOMAL-PROTEIN L1 IN XENOPUS EMBRYOS - ALTERATION OF GENE DOSAGE BY MICROINJECTION
EXPRESSION OF THE GENE FOR RIBOSOMAL-PROTEIN L1 IN XENOPUS EMBRYOS - ALTERATION OF GENE DOSAGE BY MICROINJECTION
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DOI：
10.1101/gad.2.1.23
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发表时间：
1988-01-01
影响因子：
10.5
通讯作者：
CARDINALI, B
中科院分区：
文献类型：
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作者：
PIERANDREIAMALDI, P;BOZZONI, I;CARDINALI, B
将克隆的非洲爪蟾核糖体蛋白L1基因注射到受精卵中，分析其在胚胎发育期间的表达情况，此时内源性核糖体蛋白基因产生的mrna由于翻译控制而保持沉默。注射的基因广泛复制，在胚胎中积累了10倍以上的L1成熟转录物。这伴随着少量未完全加工的L1 RNA，其中仍然含有九分之一的内含子，这是在正常条件下从未观察到的分子。多余的成熟L1 mRNA分布在多聚体和信使核糖核蛋白（mRNPs）之间，其相对比例与同期对照胚胎相同。因此，更多的L1 mRNA被装载到多体上并导致L1蛋白的出现，而这在对照胚胎中尚未检测到。结果表明，过量的L1蛋白与其转录物加工的改变之间存在关系。
Cloned gene for Xenopus ribosomal protein L1 was injected into fertilized eggs, and its expression was analyzed during the period of embryo development when the mRNAs produced by the endogenous ribosomal protein genes are still silent due to a translational control. The injected genes replicated extensively, and a 10-fold excess of L1 mature transcript accumulated in the embryo. This was accompanied by a small amount of incompletely processed L1 RNA that still contained one out of nine introns, a molecule never observed in normal conditions. The excess mature L1 mRNA was distributed between polysomes and messenger ribonucleoproteins (mRNPs) in the same relative proportion observed in control embryos of the same stage. Therefore, more L1 mRNA was loaded onto polysomes and caused the appearance of L1 protein when this was not yet detectable in control embryos. The results suggest a relationship between the excess amount of L1 protein and the alteration in processing of its transcripts.