Mass spectrometric detection of urinary oligosaccharides as markers of Schistosoma mansoni infection.
Mass spectrometric detection of urinary oligosaccharides as markers of Schistosoma mansoni infection.
复制标题
DOI：
10.1016/j.trstmh.2007.09.017
复制
发表时间：
2008
影响因子：
2.2
通讯作者：
M. L. M. Robijn-M.-L.-M.-Robijn-13696999;J. Planken;D. Kornelis;C. Hokke;A. Deelder
中科院分区：
文献类型：
--
作者：
M. L. M. Robijn-M.-L.-M.-Robijn-13696999;J. Planken;D. Kornelis;C. Hokke;A. Deelder
目前血吸虫病的诊断仍不理想。在本研究中，我们使用 mAb 114-4D12 评估了靶向亲和方法，该方法与独特的曼氏血吸虫特异性聚糖表位发生反应，并结合基质辅助激光解吸电离飞行时间 (MALDI-TOF) 质谱法。对于不同感染强度的埃及个体的 11 个尿液样本（1 毫升）中的 9 个，观察到特征性 MALDI-TOF 质谱，代表血吸虫虫卵产生的一系列岩藻寡糖。这些小分子标记物的鉴定可能会导致针对血吸虫病轻度感染的新的卵负荷相关测定。
Current diagnosis of schistosomiasis is still not ideal. In the present study we evaluated a targeted affinity approach using mAb 114-4D12, reactive with a uniqueSchistosoma mansoni-specific glycan epitope, combined with matrix-assisted laser-desorption-ionization time-of-flight (MALDI-TOF) mass spectrometry. For nine of 11 urine samples (1 ml) from Egyptian individuals with different intensities of infection, a characteristic MALDI-TOF mass spectrum was observed, representing a series of fuco-oligosaccharides that are produced by schistosome eggs. The identification of these small molecule markers may lead to a new egg-load-related assay for light infections in schistosomiasis.