Application of mtDNA sequence analysis in forensic casework for the identification of human remains
Application of mtDNA sequence analysis in forensic casework for the identification of human remains
复制标题
DOI：
10.1016/s0379-0738(00)00223-1
复制
发表时间：
2000-09-11
影响因子：
2.2
通讯作者：
Rittner, C
中科院分区：
文献类型：
--
作者：
Bender, K;Schneider, PM;Rittner, C
在去年调查的四起不明骨骼遗骸的法医案例中，我们能够将其中三起与失踪人员联系起来。在一个案例中，我们能够证明所发现的骨骼样本与一名失踪儿童不符。我们研究所通过对从法兰克福公墓获取的确定年龄的骨骼样本进行分型，建立了用于常规鉴定骨骼遗骸的线粒体DNA分析方法。对于年龄不超过75岁的骨骼，都获得了可重复的结果。为了进行分析，骨骼样本被研磨成细粉，脱钙后提取DNA。我们从DNA中扩增出线粒体DNA控制区HV - 1的一个404bp片段和HV - 2的一个379bp片段。对PCR产物进行测序后，将结果与安德森参考序列以及可能的母系亲属进行比较。（C）2000爱思唯尔科学爱尔兰有限公司。保留所有权利。
In four forensic eases of unidentified skeletal remains investigated in the last year, we were able to attach three to missing persons. In one case we could show that the discovered bone sample did not fit to a missing child. The method for mitochondrial DNA analysis for the routine identification of skeletal remains was established in our institute by typing bone samples of defined age obtained from Frankfurt's cemetery. Reproducible results were obtained for bones up to 75 years old. For analysis the bone samples were pulverised to fine powder, decalcified and DNA was extracted. From the DNA we amplified a 404-bp fragment from HV-1 and a 379-bp fragment from HV-2 of the mtDNA control region. After sequencing of the PCR products, the results were compared to the Anderson reference sequence and to putative maternal relatives. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.