The effect of 5-substituent in cytosine to the photochemical C to U transition in DNA strand
The effect of 5-substituent in cytosine to the photochemical C to U transition in DNA strand
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胞嘧啶5位取代基对DNA链光化学C到U转变的影响
DOI：
10.1016/j.bmcl.2021.127812
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发表时间：
2021
影响因子：
2.7
通讯作者：
Shigetaka Nakamura
中科院分区：
文献类型：
--
作者：
Kenzo Fujimoto;Wan Licheng;Shigetaka Nakamura
核碱基编辑是遗传疾病治疗的有力工具。我们已经报道了使用超快DNA光交联的胞嘧啶到尿嘧啶的光化学转变。在这项研究中，我们使用胞嘧啶衍生物，如甲基胞嘧啶，羟甲基胞嘧啶，和三氟甲基胞嘧啶，以评估胞嘧啶的5位取代对脱氨基的影响。胞嘧啶转化为尿嘧啶的速度最快，三氟甲基胞嘧啶转化为三氟甲基尿嘧啶的速度最慢。该顺序与含有这些胞嘧啶衍生物的双链的亲水性相关。
Nucleobase editing is a powerful tool in genetic disease therapy. We have reported the photochemical transition of cytosine to uracil using an ultrafast DNA photo-cross-linking. In this study, we used cytosine derivatives such as methylcytosine, hydroxymethylcytosine, and trifluoromethylcytosine to evaluate the effect of 5-position substitution of cytosine on deamination. The conversion of cytosine to uracil was the fastest, and the conversion of trifluoromethylcytosine to trifluoromethyluracil was the slowest. The order was correlated with the hydrophilicity of the double strand containing these cytosine derivatives.