Chorioamniontis in preterm delivery is associated with degradation of decorin and biglycan and depletion of hyaluronan in fetal membranes
Chorioamniontis in preterm delivery is associated with degradation of decorin and biglycan and depletion of hyaluronan in fetal membranes
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DOI：
10.1016/j.placenta.2014.05.004
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发表时间：
2014-08-01
期刊：
影响因子：
3.8
通讯作者：
Uldbjerg, N.
中科院分区：
文献类型：
--
作者：
Meinert, M.;Malmstroem, A.;Uldbjerg, N.
目的：蛋白多糖核心蛋白聚糖稳定胶原蛋白，而双糖蛋白聚糖和透明质酸破坏组织良好的胶原蛋白。目的是确定这些成分在胎膜中的浓度与胎龄，早产，PPROM和choriocellulonias.Study设计：早产胎膜（妊娠24-34周）从选择性剖腹产（N = 4），PPROM（N = 14），和早产（N = 14）。选择性剖腹产（N = 9）和自然阴道分娩（N = 11）的足月胎膜用于比较。组织学评估绒毛膜炎。蛋白多糖用阿新蓝沉淀、SDS-PAGE和免疫染色进行分析。通过放射免疫测定法估计Hybryonan。患有绒毛膜炎的早产羊膜显示透明质酸浓度降低8倍，核心蛋白聚糖和双糖蛋白聚糖明显降解（88（p < 0.05）。早产儿选择性剖宫产的羊膜中，（3.2对1.7 μ g/mg，p < 0.05）和双糖蛋白聚糖（0.4对1.0 μ g/mg，p <0.05）浓度，而透明质酸浓度与胎龄无关。而且，来自早产剖腹产的绒毛膜-蜕膜具有较高的核心蛋白聚糖浓度（1.8对1.0 μ g/mg，p < 0.05），而双糖蛋白聚糖浓度没有变化。劳动（术语以及早产）的特点是增加透明质酸和biglycan浓度在羊膜（无统计学意义）。结论：biglycan/核心蛋白聚糖的平衡增加在妊娠晚期和活跃的劳动。这种关系可能有助于膜的机械弱化。绒毛膜炎引起蛋白多糖和透明质酸的急剧降解，这可以解释生物力学强度的降低。(C)2014爱思唯尔有限公司版权所有。
Objective: The proteoglycan decorin stabilizes collagen whereas biglycan and hyaluronan disrupt well-organized collagen. The aim was to determine the concentrations of these constituents in fetal membranes in relation to gestational age, preterm labour, PPROM and chorioamnionitis.Study design: Preterm fetal membranes (24-34 weeks gestation) were obtained from elective caesarean deliveries (N = 4), from PPROM (N = 14), and from preterm labour (N = 14). Term fetal membranes from elective caesarean deliveries (N = 9) and spontaneous vaginal deliveries (N = 11) were used for comparison. Chorioamnionitis was assessed histologically. The proteoglycans were analysed using alcian blue precipitation, SDS-PAGE and immunostaining. Hyaluronan was estimated by a radioimmunoassay.Results: Preterm amniotic membranes with chorioamnionitis displayed a 8-fold decrease in hyaluronan concentration as well as a pronounced (88%) degradation of decorin and biglycan (p < 0.05).The amnion from preterm elective caesarean sections had higher decorin (3.2 vs. 1.7 mu g/mg, p < 0.05) and lower biglycan (0.4 vs. 1.0 mu g/mg, p < 0.05) concentrations as compared to similar term amnion (p < 0.05), whereas the hyaluronan concentrations were not associated with gestational age. Also the chorio-decidua from preterm caesarean sections had higher decorin concentrations (1.8 vs. 1.0 mu g/mg, p < 0.05) whereas the biglycan concentration was unchanged. Labour (term as well as preterm) was characterized by increased hyaluronan and biglycan concentrations in the amnion (not statistically significant).Conclusion: The biglycan/decorin balance increases during third trimester of pregnancy and during active labour. This relation might contribute to mechanical weakening of the membranes. Chorioamnionitis induces dramatic degradation of both proteoglycans and hyaluronan, which can explain the decreased biomechanical strength. (C) 2014 Elsevier Ltd. All rights reserved.