Excess polymorphism at the Adh locus in Drosophila melanogaster.
Excess polymorphism at the Adh locus in Drosophila melanogaster.
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DOI：
10.1093/genetics/114.1.93
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发表时间：
1986-09
期刊：
影响因子：
3.3
通讯作者：
M. Kreitman;Montserrat Aguadé
中科院分区：
文献类型：
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作者：
M. Kreitman;Montserrat Aguadé
通过比较黑腹果蝇（Drosophilamelanogaster）及其兄弟种果蝇（D. simulans。与D.通过使用四切割器过滤杂交技术的限制性多态性的群体调查以及通过直接序列比较来揭示黑腹菌。在这两项研究中，Adh基因包含三个编码外显子的区域表现出与4-kb 5'侧翼区相同的多态性频率。相比之下，种间序列比较显示，与结构基因座相比，5'侧翼序列的趋异水平高两倍。对变异模式的分析表明，D. Adh基因5'端侧翼区多态性缺失，而不是Adh基因5'端侧翼区多态性缺失。概述了一种方法，用于测试中性理论预测的物种内和物种之间的变化模式。这种方法表明，观察到的变化模式是不兼容的一个无限的网站中性模型。
The evolutionary history of a region of DNA encompassing the Adh locus is studied by comparing patterns of variation in Drosophila melanogaster and its sibling species, D. simulans. An unexpectedly high level of silent polymorphism in the Adh coding region relative to the 5' and 3' flanking regions in D. melanogaster is revealed by a populational survey of restriction polymorphism using a four-cutter filter hybridization technique as well as by direct sequence comparisons. In both of these studies, a region of the Adh gene encompassing the three coding exons exhibits a frequency of polymorphism equal to that of a 4-kb 5' flanking region. In contrast, an interspecific sequence comparison shows a two-fold higher level of divergence in the 5' flanking sequence compared to the structural locus. Analysis of the patterns of variation suggest an excess of polymorphism within the D. melanogaster Adh locus, rather than lack of polymorphism in the 5' flanking region. An approach is outlined for testing neutral theory predictions about patterns of variation within and between species. This approach indicates that the observed patterns of variation are incompatible with an infinite site neutral model.