A nude mouse model for the in vivo production of hepatitis B virus.
A nude mouse model for the in vivo production of hepatitis B virus.
复制标题
用于体内产生乙型肝炎病毒的裸鼠模型。
DOI：
10.1016/0016-5085(90)90840-w
复制
发表时间：
1990
期刊：
影响因子：
29.4
通讯作者：
Paronetto,F
中科院分区：
文献类型：
--
作者：
Zhai,WR;Vajta,G;Acs,G;Paronetto,F
乙型肝炎病毒基因组转染的HepG2细胞（2.2.15细胞）接种到裸鼠体内，在2-8周内产生肿瘤。血清中检测到丹恩颗粒、乙型肝炎病毒脱氧核糖核酸聚合酶活性、乙型肝炎表面抗原、乙型肝炎e抗原，36%的小鼠产生乙型肝炎核心抗原抗体。在肿瘤中，通过电子显微镜和免疫酶技术观察乙型肝炎表面、核心和e抗原。原位杂交和Southern印迹分析显示肿瘤中存在乙型肝炎病毒脱氧核糖核酸。肿瘤可以通过注射切碎的肿瘤组织或肿瘤衍生的细胞系来增殖。荷瘤小鼠的肝脏以及接种对照细胞系的小鼠的血清和组织未显示乙型肝炎病毒基因组或病毒标记。 2.2.15和未转染的HepG2细胞诱导的肿瘤均表现出菌原蛋白和各种肝癌相关抗原（α-胎蛋白、α-1-抗胰蛋白酶、α-1-抗胰凝乳蛋白酶、癌胚抗原、细胞角蛋白），表明病毒形成不会干扰这些抗原的表达。该实验模型将有助于研究药物对体内乙型肝炎病毒复制和病毒抗原表达的影响。
Hepatitis B virus genome-transfected HepG2 cells (2.2.15 cells) inoculated into nude mice produced tumors within 2–8 wk. Dane particles, hepatitis B virus deoxyribonucleic acid polymerase activity, hepatitis B surface antigen, and hepatitis B e antigen were detected in the serum, and 36% of mice developed antibodies to hepatitis B core antigen. In the tumors, hepatitis B surface, core, and e antigens were observed by electron microscopy and immunoenzymatic techniques. In-situ hybridization and Southern blot analysis showed hepatitis B virus deoxyribonucleic acid in the tumor. Tumors could be propagated by injection of minced tumor tissue or of a tumor-derived cell line. Liver of tumor-bearing mice as well as sera and tissues of mice inoculated with control cell lines did not show hepatitis B virus genome or viral markers. Tumors induced by both 2.2.15 and nontransfected HepG2 cells exhibitedmyconcogene protein and various hepatoma-associated antigens (α-fetoprotein, α-1-antitrypsin, α-1-antichymotrypsin, carcinoembryonic antigen, cytokeratin), suggesting that viral formation does not interfere with expression of these antigens. This experimental model will be helpful to study the effect of drugs on in-vivo hepatitis B virus replication and viral antigen expression.