Proteomic analysis on symbiotic differentiation of mitochondria in soybean nodules
Proteomic analysis on symbiotic differentiation of mitochondria in soybean nodules
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DOI：
10.1093/pcp/pch035
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发表时间：
2004-03-01
影响因子：
4.9
通讯作者：
Tajima, S
中科院分区：
文献类型：
--
作者：
Hoa, LTP;Nomura, M;Tajima, S
豆科植物与根瘤菌之间的共生作用诱导了根瘤中特定的代谢和胞内细胞器。为了研究大豆根瘤和根部线粒体的共生分化，采用双向电泳法比较了大豆根瘤和根部线粒体的蛋白质组成，并结合基质辅助解吸/电离飞行时间质谱仪、电喷雾电离质谱仪和N端氨基酸测序对蛋白质进行了表征。在仅在根瘤线粒体中检测到的蛋白质中，鉴定出磷酸丝氨酸转氨酶、黄烷酮3-羟基酶、辅酶原III氧化酶、1个核糖核蛋白和3个未知蛋白质。7个上调的蛋白质点，8个下调的蛋白质点和2个强烈抑制的蛋白质点也被指定为蛋白质标识。讨论了这些差异表达与大豆根瘤特定代谢相关的生理作用。
Symbiotic interactions between legume plants and rhizobia induce specific metabolisms and intracellular organelles in nodules. For surveying symbiotic differentiation of a key organelle, mitochondria, protein constituents of soybean nodule and root mitochondria were compared after two-dimensional (2-D) electrophoresis, and the proteins were characterized in combination with matrix-assisted desorption/ionization time-of-flight mass spectrometry, electrospray ionization mass spectrometry and N-terminal amino acid sequencing. Of the proteins that were detected only in nodule mitochondria, phosphoserine aminotransferase, flavanone 3-hydroxylase, coproporphyrinogen III oxidase, one ribonucleoprotein and three unknown proteins were identified. Seven up-regulated, eight down-regulated and two strongly suppressed protein spots in nodule mitochondria were also assigned protein identities. The physiological roles of these differential expressions were discussed in relation to nodule-specific metabolisms in soybean nodules.