Towards the development of a non-bioptic diagnostic technique for eosinophilic esophagitis using Raman spectroscopy
Towards the development of a non-bioptic diagnostic technique for eosinophilic esophagitis using Raman spectroscopy
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DOI：
10.1016/j.vibspec.2016.03.016
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发表时间：
2016-07-01
影响因子：
2.5
通讯作者：
Yamamoto, Tatsuyuki
中科院分区：
文献类型：
--
作者：
Noothalapati, Hemanth;Uemura, Suguru;Yamamoto, Tatsuyuki
嗜酸性食管炎（EoE）是一种以食管上皮内嗜酸性粒细胞密集浸润为特征的罕见病理状态。在过去的十年中，这种疾病在世界范围内的发生率开始迅速增加。常规内镜观察只有在观察到清晰的纵沟或多个同心环时才有助于诊断，但在早期不能给出任何明确的结论。因此，通过活组织检查从多个部位收集食管组织样本，并在染色后计数嗜酸性粒细胞的数量。这样的手术很耗时，并且有出血的固有风险，最终会损伤食管。因此，我们开发了一种基于共振拉曼光谱的方法，使用小鼠模型检测食管中的嗜酸性粒细胞。我们的研究结果表明，嗜酸性粒细胞在小鼠食管组织中的存在，患有炎症，通过管理白细胞介素-33，而没有在对照组小鼠。我们相信这种方法可以在不久的将来用于临床诊断EoE。(C)© 2016 Elsevier B. V.版权所有。
Eosinophilic esophagitis (EoE) is believed to be a rare pathological condition that is characterized by dense infiltration of eosinophils in esophageal epithelial layer. Occurrence of this disease worldwide has started increasing rapidly in the last decade. Routine endoscopic observations can help in diagnosis only if clear longitudinal furrows or multiple concentric rings are observed but does not give any definitive conclusion in the early stages. Hence esophageal tissue samples are collected from multiple sites by biopsy and the number of eosinophils is counted after staining. Such a procedure is time consuming and has an inherent risk of bleeding, eventually damaging esophagus. Hence we developed a resonance Raman spectroscopy based approach to detect eosinophils in esophagus using mouse models. Our results show the presence of eosinophils in mice esophageal tissues suffering from inflammation by administering interleukin-33 while there are none in control mice. We believe this method can be used in clinical application for diagnosing EoE in the near future. (C) 2016 Elsevier B.V. All rights reserved.