Ionized gas (plasma) delivery of reactive oxygen species (ROS) into artificial cells
Ionized gas (plasma) delivery of reactive oxygen species (ROS) into artificial cells
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DOI：
10.1088/0022-3727/47/36/362001
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发表时间：
2014-09-10
影响因子：
3.4
通讯作者：
Short, Robert D.
中科院分区：
文献类型：
--
作者：
Hong, Sung-Ha;Szili, Endre J.;Short, Robert D.
这项研究旨在增强我们对电离气体（等离子体）异位产生的活性氧（ROS）如何影响细胞内信号传导过程的调控的理解。一个模型系统，包括一个悬浮液的磷脂囊泡（细胞模拟物）封装的ROS报告，被开发来研究ROS进入细胞的血浆递送。这是第一次表明，血浆明确地提供ROS进入细胞在一个持续的时间，而不损害细胞膜的完整性。细胞和生物测定中的一个重要考虑因素是血清的存在，其显著降低了ROS向囊泡中的转移效率。这些结果对于理解等离子体治疗如何针对特定的医疗或生物技术应用是关键的。此外，磷脂囊泡ROS报告系统可能会发现在其他研究中使用涉及自由基在生物学和医学中的应用。
This study was designed to enhance our understanding of how reactive oxygen species (ROS), generated ex situ by ionized gas (plasma), can affect the regulation of signalling processes within cells. A model system, comprising of a suspension of phospholipid vesicles (cell mimics) encapsulating a ROS reporter, was developed to study the plasma delivery of ROS into cells. For the first time it was shown that plasma unequivocally delivers ROS into cells over a sustained period and without compromising cell membrane integrity. An important consideration in cell and biological assays is the presence of serum, which significantly reduced the transfer efficiency of ROS into the vesicles. These results are key to understanding how plasma treatments can be tailored for specific medical or biotechnology applications. Further, the phospholipid vesicle ROS reporter system may find use in other studies involving the application of free radicals in biology and medicine.