Hair cell recovery in the chinchilla crista ampullaris after gentamicin treatment:: A quantitative approach
Hair cell recovery in the chinchilla crista ampullaris after gentamicin treatment:: A quantitative approach
复制标题
DOI：
10.1016/s0194-5998(98)70060-9
复制
发表时间：
1998-09-01
影响因子：
3.4
通讯作者：
Beykirch, K
中科院分区：
文献类型：
--
作者：
López, I;Honrubia, V;Beykirch, K
在将50μg庆大霉素注入龙猫上半规管的外淋巴间隙后，对毛细胞恢复的机制进行了研究。采用光镜和透射电镜技术对一个正常组和四个治疗后组（7天、14天、28天和56天）进行了组织学评估。用切割器技术对毛细胞和支持细胞的数量变化进行了定量。治疗后7天和14天，没有I型毛细胞存在，II型毛细胞分别丢失了85%和88%。支持细胞在7天减少到76%，但在14天恢复到91%。治疗后28天上皮细胞明显恢复；83%为II型毛细胞，3%为I型毛细胞。支持细胞数量接近正常（86%）。在治疗后14天到28天之间，II型毛细胞增加了1758个，大约每天新增125个毛细胞。在同一时间间隔内，支持细胞数量保持接近正常。这些结果表明，新的毛细胞可能是支持细胞有丝分裂和分化的结果。
The mechanisms for hair cell recovery were investigated after intraotic application of 50,mu g gentamicin into the perilymphatic space of the superior semicircular canal of the chinchilla. Histologic evaluation of one normal group and four posttreatment groups (7, 14, 28, and 56 days) was made with light and transmission electron microscopic techniques. The numeric changes of hair cells and supporting cells was quantified with the dissector technique. At 7 and 14 days after treatment, no type I hair cells were present, and 85% and 88% of type II hair cells were lost. Supporting cells decreased to 76% at 7 days, but they recovered to 91% at 14 days. Recovery of the epithelia was evident 28 days after treatment; 83% were type II hair cells, and 3% were type I hair cells. The supporting cell number remained close to normal (86%). Between 14 and 28 days after treatment, there was an increase of 1758 of type II hair cells, representing approximately 125 new hair cells per day. At the same time interval the number of supporting cells remained near normal. These results suggest that new hair cells might be the result of supporting cell mitotic division and differentiation.