Distribution of the glutamate transporters GLT-1 (SLC1A2) and GLAST (SLC1A3) in peripheral organs
Distribution of the glutamate transporters GLT-1 (SLC1A2) and GLAST (SLC1A3) in peripheral organs
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DOI：
10.1007/s00429-006-0109-x
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发表时间：
2006-11-01
期刊：
影响因子：
--
通讯作者：
Hediger, Matthias A.
中科院分区：
文献类型：
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作者：
Berger, Urs V.;Hediger, Matthias A.
谷氨酸转运体GLT-1和GLAST广泛表达于脑中的星形胶质细胞中，它们在谷氨酸能神经传递过程中履行重要功能。本研究采用原位杂交（ISH）和免疫细胞化学方法检测其在外周器官中的分布。发现GLAST比GLT-1分布更广泛。GLAST主要在上皮细胞、巨噬细胞系细胞、淋巴细胞、脂肪细胞、间质细胞和唾液腺腺泡中表达。GLT-1主要在腺体组织中表达，包括乳腺、泪腺和唾液腺中的导管和腺泡，但也在脾和淋巴结中的静脉周肝细胞和滤泡树突状细胞中表达。研究结果表明，虽然在大脑中由相同的细胞表达，但这两种谷氨酸转运蛋白在外周组织中具有不同的分布模式，并且它们在这些区域中除了谷氨酸能神经传递之外还实现谷氨酸转运功能。
The glutamate transporters GLT-1 and GLAST are widely expressed in astrocytes in the brain where they fulfill important functions during glutamatergic neurotransmission. The present study examines their distribution in peripheral organs using in situ hybridization (ISH) and immunocytochemistry. GLAST was found to be more widely distributed than GLT-1. GLAST was expressed primarily in epithelial cells, cells of the macrophage-lineage, lymphocytes, fat cells, interstitial cells, and salivary gland acini. GLT-1 was primarily expressed in glandular tissue, including mammary gland, lacrimal gland, and ducts and acini in salivary glands, but also by perivenous hepatocytes and follicular dendritic cells in spleen and lymph nodes. The findings demonstrate that, although expressed by the same cells in the brain, these two glutamate transporters have different distribution patterns in peripheral tissues and that they fulfill glutamate transport functions apart from glutamatergic neurotransmission in these areas.