Circadian modulation of dopamine receptor responsiveness in Drosophila melanogaster
Circadian modulation of dopamine receptor responsiveness in Drosophila melanogaster
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DOI：
10.1073/pnas.97.4.1873
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发表时间：
2000-02-15
影响因子：
11.1
通讯作者：
Hirsh, J
中科院分区：
文献类型：
--
作者：
Andretic, R;Hirsh, J
我们研究了果蝇多巴胺受体的昼夜节律功能，通过使用行为活跃的去头制剂，允许直接将药物应用于神经索。喹匹罗是一种d2样多巴胺受体激动剂，可诱导无头苍蝇的反射运动。我们表明，运动诱导的数量变化是一天中时间的函数，在主观夜间对喹匹罗的反应最高。此外，多巴胺受体的反应受昼夜节律控制，取决于周期基因的正常功能。头部起搏器对于喹匹罗诱导的运动的昼夜节律调节至少在一定程度上是不可缺少的，因为在被斩首的果蝇衰老12小时后，激动剂反应性的变化仍然存在。这一发现表明，体内周期依赖的分子振荡器在胺受体反应性的调节中发挥了作用。
We investigated the circadian function of Drosophila dopamine receptors by using a behaviorally active decapitated preparation that allows for direct application of drugs to the nerve cord. Quinpirole, a D2-like dopamine receptor agonist, induces reflexive locomotion in decapitated flies. We show that the amount of locomotion induced changes as a function of the time of day, with the highest responsiveness to quinpirole during the subjective night. Furthermore, dopamine receptor responsiveness is under circadian control and depends on the normal function of the period gene. The head pacemaker is at least partly dispensable for the circadian modulation of quinpirole-induced locomotion, because changes in agonist responsiveness persist in decapitated flies that are aged for 12 h, This finding suggests a role for the period-dependent molecular oscillators in the body in the modulation of amine receptor responsiveness.