Increased hepcidin expression in non-small cell lung cancer tissue and serum is associated with clinical stage
Increased hepcidin expression in non-small cell lung cancer tissue and serum is associated with clinical stage
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非小细胞肺癌组织和血清中铁调素表达增加与临床分期相关
DOI：
10.1111/1759-7714.12046
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发表时间：
2014-01-01
期刊：
影响因子：
2.9
通讯作者：
Yu, Fenglei
中科院分区：
文献类型：
--
作者：
Chen, Qian;Wang, Li;Yu, Fenglei
背景铁调素是一种分泌性小肽，在铁代谢中起关键作用。高水平的hepcidin表达可能与结直肠癌有关;然而，hepcidin和肺癌之间的关系尚未研究。MethodsSerum hepcidin-25，骨形态发生蛋白（BMP）-2，IL-6浓度在53例患者和16例非癌个体测定酶联免疫吸附试验。应用逆转录定量聚合酶链反应（RT-qPCR）检测65例非小细胞肺癌（NSCLC）患者手术切除标本中癌组织和非癌组织、6种肺癌细胞系和人支气管上皮（HBE）细胞中hepcidin mRNA的表达。结果非小细胞肺癌患者血清hepcidin-25浓度明显高于非癌患者，且与血清BMP-2浓度呈正相关，与血清IL-6浓度呈负相关。血清hepcidin水平与淋巴结转移和临床分期有关。Hepcidin mRNA在NSCLC癌组织中的表达高于正常肺组织（P = 0.001）。Hepcidin mRNA在4种肺癌细胞系中的表达均高于HBE细胞。免疫组化结果显示，hepcidin蛋白在NSCLC癌组织中表达增高。非小细胞肺癌患者血清hepcidin-25水平与淋巴结转移和肿瘤临床分期有关。
BackgroundHepcidin is a small secreted peptide that plays a key role in iron metabolism. A high level of hepcidin expression may be implicated in colorectal cancer; however, the relationship between hepcidin and lung cancer has not yet been studied.MethodsSerum hepcidin-25, bone morphogenetic protein (BMP)-2, and interleukin (IL)-6 concentration in 53 patients and 16 non-cancerous individuals was measured by enzyme-linked immune sorbent assay. Reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) was utilized to study the expression of hepcidin mRNA in paired tumor and non-tumor lung tissues in surgical specimens from 65 patients with non small cell lung cancer (NSCLC), as well as in six types of lung cancer cell lines and human bronchial epithelial (HBE) cells. Hepcidin protein expression and cellular localization in NSCLC was determined by immunohistochemistry.ResultsThe serum hepcidin-25 concentration was higher in patients with NSCLC than in non-cancerous individuals, and was positively correlated with serum BMP2 concentration, but negatively with serum IL-6 levels. Serum hepcidin was also correlated with lymph node metastasis and clinical stage. Hepcidin mRNA expression was higher in cancerous tissues of NSCLC than in normal pulmonary tissues (P = 0.001). Hepcidin mRNA levels in four lung carcinoma cell lines were higher than in HBE cells. Immunohistochemistry showed that hepcidin protein was increased in cancerous tissues of NSCLC.ConclusionsThe level of hepcidin expression increased in NSCLC tissue and serum. Serum hepcidin-25 level was associated with lymph node metastasis and tumor clinical stage in patients with NSCLC.