Dentin tubule obturation of a bioglass-based dentin desensitizer under repeated exposure to lactid acid and brushing
Dentin tubule obturation of a bioglass-based dentin desensitizer under repeated exposure to lactid acid and brushing
复制标题
DOI：
10.1186/s12903-019-0962-7
复制
发表时间：
2019-12-05
期刊：
影响因子：
2.9
通讯作者：
Sahrmann, Philipp
中科院分区：
文献类型：
--
作者：
Manz, Andrea S.;Attin, Thomas;Sahrmann, Philipp
背景：牙本质过敏是一种常见的发现，特别是在牙周炎患者。传统治疗的目的是通过禁止液体和渗透波动进出牙髓腔来阻塞牙本质小管。一种新的基于滑石的脱敏剂被证明可以阻塞小管并抵抗定期暴露于乳酸。到目前为止，还没有测试过这种阻塞物是否耐刷。方法：将60个牙本质盘用17%EDTA清洗后，放入牙髓液模拟器中，随机分为3组：A组不进行表面处理，B组使用Seal&Protect（R），C组使用DentinoCer。椎间盘暴露于0.1 M不饱和乳酸三次，每天标准化刷牙两次，持续12天。在基线和2、4和12天后，将样品从环境中取出，并准备用于顶视图SEM分析，以使用Olley评分评估小管阻塞。然后垂直切割椎间盘，并使用背散射成像对切片表面进行形态学评估。对于垂直和截面表面，EDX分析被用来表征在肾小管和肾小管间areas.Results的表面组成：A组显示在所有时间点的清洁肾小管腔。从第2天开始，牙本质显示出暴露的胶原纤维。第2组最初显示出完整的表面覆盖，在处理期间变平，从未暴露小管。在基线时，C组样本显示完全均匀覆盖。从第2天开始，可见管腔阻塞的小管入口。第4天和第12天牙本质表面胶原纤维暴露，牙本质腔仍保持封闭。X射线能谱分析表明，P、Ca是牙本质间和牙本质小管中的主要元素，Si在牙本质小管栓中出现峰。结论：B组牙本质表面在整个观察时间内均为致密层，C组牙本质表面在48 h内消失。小管塞含有相当大的硅比例表明以前存在的DentinoCer，而高钙和磷的比例表明闭塞的牙本质样材料。
Background: Dentin hypersensitivity is a frequent finding especially in periodontitis patients. Conventional treatment aims for obstruction of dentin tubules by disabling liquid and osmotic fluctuation to and from the pulpal chamber. A novel bioglass-based desensitizer was shown to obstruct tubules and to resist periodic exposure to lactic acid. Whether this obstruction is resistant to brushing had not been tested so far. Accordingly, the present study aimed to assess dentin tubule obstruction after repeated acid exposure and brushing.Methods: Sixty dentin discs were cleaned with 17% EDTA, mounted into a pulp fluid simulator and randomly divided into 3 groups: No surface treatment in Group A, Seal&Protect (R) in group B and DentinoCer in group C. Discs were exposed to 0.1 M non-saturated lactic acid thrice and standardized brushing twice a day for 12 days. At baseline and after 2, 4 and 12 d samples were removed from the setting and prepared for top-view SEM analysis to assess tubule obstruction using the Olley score. Discs were then vertically cut and the section surface morphologically assessed using backscatter imaging. For both vertical and sectional surfaces EDX analysis was used to characterize the surface composition in the tubular and inter-tubular area.Results: Group A showed clean tubular lumina at all time points. From day 2 onwards dentin showed exposed collagen fibers. Group 2 initially showed a complete surface coverage that flattened out during treatment without ever exposing tubules. At baseline, samples of Group C displayed a complete homogeneous coverage. From day 2 on tubules entrances with obstructed lumen became visible. While on day 4 and 12 the dentin surface exposed collagen fibers the lumina remained closed. EDX analysis of the vertical and horizontal views showed that P and Ca were predominant elements in both the inter- and tubular dentin while Si peaks were found in the tubule plugs.Conclusion: While group B displayed a packed layer on the surface during the whole investigation time group C samples lost their superficial layer within 48 h. Tubule plugs containing considerable Si proportions indicated previous presence of DentinoCer, while high Ca and P proportions suggest obturation by dentin-like material.