Antifibrotic effects of a herbal combination regimen on hepatic fibrotic rats
Antifibrotic effects of a herbal combination regimen on hepatic fibrotic rats
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DOI：
10.1002/ptr.2265
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发表时间：
2008-01-01
影响因子：
7.2
通讯作者：
Huang, Yi-Tsau
中科院分区：
文献类型：
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作者：
Lin, Yun-Lian;Hsu, Yi-Chao;Huang, Yi-Tsau
肝纤维化是一种涉及多种分子致病途径的慢性炎症过程。本研究旨在探讨丹参、川芎和光甘草联合用药对肝纤维化大鼠的体内抗纤维化作用。通过给予二甲基亚硝胺（DMN）4周诱导大鼠纤维化。将纤维化大鼠随机分配到三个组中的一个：对照组、SLG（50 mg/kg）或水飞蓟素（50 mg/kg），从DMN注射后1周开始，每天两次通过管饲法给予每个组，持续3周。结果显示，与接受媒介物的DMN处理的大鼠（1.63 +/-0.18）相比，用SLG处理的DMN处理的大鼠（1.13 +/-0.13）的肝脏纤维化评分显著降低。此外，无论是SLG或水飞蓟素治疗DMN大鼠肝胶原含量显着降低。免疫组化双重染色结果也显示，SLG和水飞蓟素治疗后，纤维化肝脏中NF κ B核转位的α-SMA阳性细胞减少。SLG和水飞蓟素处理可降低TGF-β 1、α-SMA、胶原1 α 2、iNOS和ICAM-1基因的mRNA表达水平。结果表明，SLG对DMN诱导的大鼠肝纤维化有明显的抗纤维化作用。版权所有（c）2007约翰威利父子有限公司。
Liver fibrosis has been characterized as chronic inflammatory processes involving multiple molecular pathogenetic pathways. This therapeutic study investigated whether a combination regimen of Salvia miltiorrhiza (S), Ligusticum chuanxiong (L) and Glycyrrhiza glabra (G) exerted in vivo antifibrotic effects on rats with hepatic fibrosis. Fibrosis was induced in rats by dimethylnitrosamine (DMN) administration for 4 weeks. Fibrotic rats were randomly assigned to one of the three groups: control, SLG (50 mg/kg) or silymarin (50 mglkg), each given by gavage twice daily for 3 weeks starting 1 week after DMN injection. The results showed that fibrosis scores of livers from DMN-treated rats with SLG (1.13 +/- 0.13) were significantly reduced in comparison with DMN-treated rats receiving vehicle (1.63 +/- 0.18). Moreover, the hepatic collagen content of DMN rats was significantly reduced by either SLG or silymarin treatment. The double immunohistochemical staining results also showed that alpha-SMA positive cells with NF kappa B nuclear translocation were decreased in the fibrotic livers by SLG and silymarin treatments. The mRNA expression levels of TGF-beta 1, alpha-SMA, collagen1 alpha 2, iNOS and ICAM-1 genes were attenuated by SLG and silymarin treatment. The results showed that SLG exerted antifibrotic effects in rats with DMN-induced hepatic fibrosis. Copyright (c) 2007 John Wiley & Sons, Ltd.