Hunt for pluripotent stem cell -- regenerative medicine search for almighty cell.
Hunt for pluripotent stem cell -- regenerative medicine search for almighty cell.
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DOI：
10.1016/j.jaut.2007.12.003
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发表时间：
2008-05
影响因子：
12.8
通讯作者：
M. Ratajczak;E. Zuba-Surma;M. Wysoczynski;Wu Wan;J. Ratajczak;W. Wojakowski;M. Kucia
中科院分区：
文献类型：
--
作者：
M. Ratajczak;E. Zuba-Surma;M. Wysoczynski;Wu Wan;J. Ratajczak;W. Wojakowski;M. Kucia
再生医学和组织工程正在寻找一种基于干细胞的新型治疗策略，该策略将允许有效治疗甚至可能替代受损器官。多能干细胞（PSC）可产生所有三种生殖谱系的细胞，似乎是此类疗法最理想的候选者。 PSC 可以从发育中的胚胎中提取。然而，由于这种用于潜在治疗目的的干细胞来源仍然存在争议，干细胞研究人员寻找可以从成体组织中分离出来或从已经分化的细胞中产生的 PSC。真正的 PSC 应该具有体外多谱系分化的潜力，更重要的是，还能够补充体内囊胚的发育。这篇综述将总结目前从成体组织中分离 PSC 或通过从已分化的体细胞进行核重编程产生 PSC 的方法和局限性。
Regenerative medicine and tissue engineering are searching for a novel stem cell based therapeutic strategy that will allow for efficient treatment or even potential replacement of damaged organs. The pluripotent stem cell (PSC), which gives rise to cells from all three germ lineages, seems to be the most ideal candidate for such therapies. PSC could be extracted from developing embryos. However, since this source of stem cells for potential therapeutic purposes remains controversial, stem cell researchers look for PSC that could be isolated from the adult tissues or generated from already differentiated cells. True PSC should possess both potential for multilineage differentiation in vitro and, more importantly, also be able to complement in vivo blastocyst development. This review will summarize current approaches and limitations to isolate PSC from adult tissues or, alternatively, to generate it by nuclear reprogramming from already differentiated somatic cells.