Quantitative analysis of human enteric adenoviruses in aquatic environments
Quantitative analysis of human enteric adenoviruses in aquatic environments
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DOI：
10.1111/j.1365-2672.2007.03453.x
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发表时间：
2007-12-01
影响因子：
4
通讯作者：
Ohgaki, S.
中科院分区：
文献类型：
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作者：
Haramoto, E.;Katayama, H.;Ohgaki, S.
目的：本研究的目的是确定人类腺病毒（HuAdVs）在水生环境中通过实时聚合酶链反应（PCR.Methods和Results）：为了描述肠道血清型的比例，总HuAdVs，特异性的引物组肠道血清型40和41平行使用的通用引物组的所有51个血清型的HuAdVs。在原水（n = 17）中检测到7.3-1500个PCR检测单位（PDU）/ml的HuAdV肠道血清型，在氯化前的二级处理污水（n = 17）中检测到0.00060-4.1 PDU ml（-1）的HuAdV肠道血清型，在河水（n = 36）中检测到0.0018-7.0 PDU ml（-1）的HuAdV肠道血清型，海水中0.032- 6.1PDU ml（-1）（n = 18）。结论：水环境中HuAdV的血清型以肠道型为主;研究意义和影响：在水环境中HuAdV的血清型中，肠道型HuAdV的丰度比其他血清型的丰度更高，以评估其经水感染的风险。
Aims: The aim of this study was to determine human adenoviruses (HuAdVs) in aquatic environments by real-time polymerase chain reaction (PCR).Methods AND Results: In order to describe the ratio of enteric serotypes to the total HuAdVs, the primer set specific for the enteric serotypes 40 and 41 was used in parallel with the universal primer set for all 51 serotypes of HuAdVs. The enteric serotypes of HuAdVs were detected at the concentration of 7.3-1500 PCR-detection units (PDU) per ml in raw sewage (n = 17), 0.00060-4.1 PDU ml(-1) in secondary-treated sewage before chlorination (n = 17), 0.0018-7.0 PDU ml(-1) in river water (n = 36), and 0.032-6.1 PDU ml(-1) in seawater (n = 18). The concentration of HuAdVs, determined by the universal primer set, was equivalent to that of enteric serotypes in almost all the samples tested.Conclusion: Enteric serotypes were predominant among all serotypes of HuAdVs in the aquatic environments.Significance and the Impact of the Study: The abundance of enteric serotypes of HuAdVs should be more emphasized than other serotypes in order to assess the risk of their infection via water.