Skeletal muscle regeneration after insulin-like growth factor I gene transfer by recombinant Sendai virus vector
Skeletal muscle regeneration after insulin-like growth factor I gene transfer by recombinant Sendai virus vector
复制标题
重组仙台病毒载体转胰岛素样生长因子I基因后骨骼肌再生
作者：
A. Shiotani;M. Fukumura;M. Maeda;X. Hou;M. Inoue;T. Kanamori;S. Komaba;K. Washizawa;S. Fujikawa;T. Yamamoto;C. Kadono;K. Watabe;H. Fukuda;K. Saito;Y. Sakai;Y. Nagai;J. Kanzaki;M. Hasegawa
我们考察了表达LacZ或人胰岛素样生长因子- 1 （hIGF-I）的仙台病毒（SeV）载体在骨骼肌基因转移中的适用性和有效性。布比卡因治疗前后，大鼠胫骨前肌肌内注射LacZ/SeV X-gal标记肌纤维在注射后第7天出现，转基因表达持续至注射后1个月。在新生大鼠成肌细胞系L6的培养上清液中检测到重组higf - 1作为主要蛋白种，诱导细胞发生肌发生分化。与LacZ/SeV处理的对照肌肉相比，将hIGF-I/SeV引入肌肉后，肌纤维的再生和分裂显著增加，这表明肌纤维肥大，肌纤维总数也有所增加。这些结果表明，SeV在骨骼肌中实现了高水平的转基因表达，因此，利用SeV载体转移hIGF-I基因在神经肌肉疾病的治疗中可能具有很大的潜力。
We scrutinized the applicability and efficacy of Sendai virus (SeV) vectors expressing either LacZ or human insulin-like growth factor-I (hIGF-I) in gene transfer into skeletal muscle. Seven days after the intramuscular injection of LacZ/SeV X-gal labeled myofibers were demonstrated in rat anterior tibialis muscle with/without bupivacaine treatment and the transgene expression persisted up to 1 month after injection. Recombinant hIGF-I was detected as a major protein species in culture supernatants of a neonatal rat myoblast cell line L6 and thus induced the cells to undergo myogenetic differentiation. The introduction of hIGF-I/SeV into the muscle showed a significant increase in regenerating and split myofibers which were indicative of hypertrophy, and also an increase in the total number of myofibers, in comparison to that seen in the LacZ/SeV-treated control muscle. These results demonstrate that SeV achieves high-level transgene expression in skeletal muscle, and that hIGF-I gene transfer using SeV vector may therefore have great potential in the treatment of neuromuscular disorders.
通过肌细胞生成素 mRNA 的反义寡脱氧核糖核苷酸高度特异性抑制 IGF-I 刺激的分化。
DOI：
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发表时间：
1990
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Florini,JR;Ewton,DZ
通讯作者：
Ewton,DZ
DOI：
10.1073/pnas.95.26.15603
发表时间：
1998-12-22
影响因子：
11.1
作者：
Barton-Davis, ER;Shoturma, DI;Sweeney, HL
通讯作者：
Sweeney, HL