Identification of a crystal cell-specific enhancer of the black cells prophenoloxidase gene in drosophila
Identification of a crystal cell-specific enhancer of the black cells prophenoloxidase gene in drosophila
复制标题
DOI：
10.1002/dvg.20285
复制
发表时间：
2007-04-01
期刊：
影响因子：
1.5
通讯作者：
Schulz, Robert A.
中科院分区：
文献类型：
--
作者：
Gajewski, Kathleen M.;Sorrentino, Richard P.;Schulz, Robert A.
在果蝇中，黑细胞（Bc）编码一种过氧化物酶，并在晶体细胞成熟后期表达，晶体细胞是参与伤口愈合和免疫包囊的血细胞。Bc的增强子分析揭示了1，025-bp的上游序列，其以晶体细胞排他模式调节基因表达。该片段的表达因加塔家族蛇蛋白（srp）和RUNX家族菱形蛋白（lz）基因突变而改变; Srp和Lz是晶体细胞特化所需的。缺失分析揭示了一个330 bp的晶体细胞特异性序列，其中包含两个加塔和三个Lz结合位点。突变分析表明，这两个加塔网站是必要的，但不足以为晶体细胞的表达。然而，其中一个Lz位点对于晶体细胞表达是必需的。因此，Srp和Lz不仅指定晶体细胞谱系，而且还调节这些细胞的后期分化。此外，我们现在有一个敏感的工具来标记活体动物的晶体细胞。
In Drosophila, Black cells (Bc) encodes a Propheholoxidase and is expressed late in the maturation of crystal cells, which are blood cells involved in wound healing and immune encapsulation. Enhancer analysis of Bc revealed a 1,025-bp upstream sequence that regulates gene expression in a crystal cell exclusive pattern. Expression of this fragment is altered by mutations in the GATA family serpent (srp) and RUNX family lozenge (lz) genes; Srp and Lz are required for crystal cell specification. Deletional analysis uncovered a 330-bp crystal cell-specific sequence, which contains two GATA and three Lz binding sites. Mutational analysis revealed that both GATA sites are necessary, but not sufficient for crystal cell expression. However, one of the Lz sites is essential for crystal cell expression. Thus, Srp and Lz do not just specify the crystal cell lineage, but also regulate the later differentiation of these cells. Additionally, we now have a sensitive tool for marking crystal cells in live animals.