Studies of insulin resistance in adipocytes induced by macrophage mediator.
Studies of insulin resistance in adipocytes induced by macrophage mediator.
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DOI：
10.1084/jem.157.4.1360
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发表时间：
1983-04-01
期刊：
影响因子：
--
通讯作者：
Cerami A
中科院分区：
文献类型：
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作者：
Pekala P;Kawakami M;Vine W;Lane MD;Cerami A
在加入内毒素诱导的巨噬细胞介体后，3T3-L1脂肪细胞出现明显的胰岛素抵抗。胰岛素受体水平的检测表明，该介体既不影响细胞结合胰岛素的功能能力，也不影响胰岛素刺激葡萄糖摄取的能力。这种抗性似乎反映了受体后对胰岛素诱导的合成酶、乙酰辅酶A羧基酶和脂肪酸合成酶的生物合成的干扰，这两种酶是将葡萄糖转化为储存脂所必需的。这些研究为研究胰岛素抵抗的分子基础提供了一种新的体外模型。
An apparent insulin resistance is noted in 3T3-L1 adipocytes after the addition of an endotoxin-induced mediator from macrophages. Examination at the level of the insulin receptor has revealed that the mediator does not effect either the functional ability of the cells to bind insulin or the ability of insulin to stimulate the uptake of glucose. The resistance appears to reflect a post-receptor interference with the insulin-induced biosynthesis of the anabolic enzymes, acetyl Co-A carboxylase and fatty acid synthetase, which are required for the conversion of glucose into storage lipid. These studies offer a new in vitro model for investigating the molecular basis of insulin resistance.