Analysis of expression of human mammary epithelial antigens in normal and malignant breast cells at the single cell level by flow cytofluorimetry.
Analysis of expression of human mammary epithelial antigens in normal and malignant breast cells at the single cell level by flow cytofluorimetry.
复制标题
通过流式细胞荧光法在单细胞水平分析正常和恶性乳腺细胞中人乳腺上皮抗原的表达。
DOI：
10.1159/000163773
复制
发表时间：
1981
期刊：
影响因子：
--
通讯作者：
Ceriani,RL
中科院分区：
文献类型：
--
作者：
Peterson,JA;Bartholomew,JC;Stampfer,M;Ceriani,RL
通过流式细胞荧光测定法在单细胞水平上分析人乳腺上皮细胞表面上的细胞类型特异性抗原（HME抗原）的表达。描述了处理两个技术难题的方法，这两个技术难题阻碍了通过流式细胞荧光测定法对来自原代培养物的细胞上的表面抗原进行定量，即细胞凝集和几种细胞类型的存在。通过用两种不同的荧光标记物同时染色细胞：用荧光素标记的抗体（绿色荧光）染色细胞表面HME抗原和用碘化丙啶（红色荧光）染色细胞DNA，可以确定每单位DNA的HME抗原的表达，分析它们在混合群体中的乳腺细胞上的表达，并分析它们在细胞周期的不同阶段中的表达。HME抗原减少恶性乳腺上皮细胞相比，他们的正常对应。
The expression of cell-type specific antigens on the surface of human mammary epithelial cells (HME antigens) is analyzed at the single cell level by flow cytofluorimetry. Methods are described to deal with two technical difficulties that have hampered the quantitation of surface antigens on cells from primary cultures by flow cytofluorimetry, namely, cell clumping and the presence of several cell types. By staining the cells simultaneously with two different fluorescent markers: the cell surface HME antigens with fluorescein-tagged antibodies (green fluorescence) and the cell DNA with propidium iodide (red fluorescence), it was possible to determine the expression of HME antigens per unit DNA, analyze their expression on breast cells in mixed populations, and analyze their expression in different phases of the cell cycle. The HME antigens were diminished in malignant breast epithelial cells as compared to their normal counterpart.