Replication of flock house virus in three genera of medically important insects
Replication of flock house virus in three genera of medically important insects
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DOI：
10.1603/0022-2585(2007)44
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发表时间：
2007-01-01
影响因子：
2.1
通讯作者：
Christensen, Bruce M.
中科院分区：
文献类型：
--
作者：
Dasgupta, Ranjit;Free, Heather M.;Christensen, Bruce M.
鸡群屋病毒（Flock House Virus，FHV）是从新西兰产的玉米粉蚧（Costelytra zealandica，白色）（鞘翅目：金龟子科）中分离到的一种基因组分离的正义RNA昆虫病毒。FHV在昆虫、线虫、植物和酵母中复制。我们先前报道了FHV在四个属的蚊子中的复制和绿色荧光蛋白在埃及伊蚊（L.）由基于FHV的载体产生。我们在这里报告说，FHV在疟疾媒介冈比亚按蚊Giles和An体内繁殖旺盛。stephensi Liston和体外在源自An.冈比亚。此外，FHV在其他两种重要的医学昆虫中广泛繁殖，采采蝇Glossina morsitans morsitans Westwood和红蝽Rhodnius prolixus Stal，将其宿主范围扩展到四个昆虫目（鞘翅目，鳞翅目，双翅目和半翅目）。这种病毒在所研究的昆虫的所有主要组织中传播。当FHV以高于10（4）个噬斑形成单位（pfu）的剂量注射或病毒累积至滴度高于10（8）pfu时，按蚊和Glossina显示死亡率。较低剂量（10（3）pfu）促进更广泛的病毒增殖，并将死亡率降低至<10%。在Ae中未观察到不良反应。埃及库蚊，库蚊，和暗翅阿蚊（Armigeres subalbatus）（Coquillett），当注射高达10（7）pfu的剂量时。经口饲喂FHV的蚊虫表现出较慢的病毒生长速率和较低的死亡率。我们的研究结果表明，FHV具有独特的广泛的昆虫宿主范围，该病毒可用于研究病毒在各种医学上重要的昆虫宿主相互作用。
Flock House Virus (family Nodaviridae, genus Alphanodavirus, FHV) was originally isolated from grass grubs Costelytra zealandica (White) (Coleoptera: Scarabaeidae) in New Zealand and belongs to a family of divided genome, plus-sense RNA insect viruses. FHV replicates in insects, a nematode, plants, and yeast. We previously reported replication of FHV in four genera of mosquitoes and expression of green fluorescent protein in Aedes aegypti (L.) produced by an FHV-based vector. We report here that FHV multiplies vigorously in vivo in the malaria vectors Anopheles gambiae Giles and An. stephensi Liston and in vitro in a cell line derived from An. gambiae. In addition, FHV multiplies extensively in two other medically important insects, the tsetse fly, Glossina morsitans morsitans Westwood, and the reduviid bug Rhodnius prolixus Stal, extending its host range to four orders of insects (Coleoptera, Lepidoptera, Diptera, and Hemiptera). The virus disseminates in all the major tissues of the insects studied. Anopheles and Glossina show mortality when FHV is injected at a dose above 10(4) plaque-forming units (pfu) or the virus accumulates to titer above 10(8) pfu. A lower dose (10(3) pfu) promotes more extensive virus multiplication and reduces mortality to < 10%. No adverse effects are observed in Ae. aegypti, Culex pipiens pipiens L., and Armigeres subalbatus (Coquillett), when injected with a dose of up to 10(7) pfu. Mosquitoes orally fed with FHV exhibited slower virus growth rate with lower mortality. Our results indicate that FHV has uniquely broad insect host range and that the virus can be used to study virus host interactions in a variety of medically important insects.