Tissue-specific accumulation of pH-sensing phosphatidic acid determines plant stress tolerance
Tissue-specific accumulation of pH-sensing phosphatidic acid determines plant stress tolerance
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pH 敏感磷脂酸的组织特异性积累决定植物的胁迫耐受性
DOI：
10.1038/s41477-019-0497-6
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发表时间：
2019-09-01
期刊：
影响因子：
18
通讯作者：
Zhang, Wenhua
中科院分区：
文献类型：
--
作者：
Li, Wenyu;Song, Tengzhao;Zhang, Wenhua
信号脂质磷脂酸（PA）参与调节植物的各种基本生物学过程。然而，PA行动的机制仍然知之甚少，因为目前可用的方法监测PA未能确定精确的时空动态的信使在活细胞和组织的植物。在这里，我们开发了PAleon，一种PA特异性光遗传学生物传感器，基于Förster共振能量转移（FRET）报告了质膜上生物活性PA的浓度和动力学。PAleon是足够敏感，以监测生理浓度的PA在活细胞中，并可视化PA动态在亚细胞分辨率的组织时，他们受到挑战与脱落酸（阿坝）和盐胁迫。Paleon生物成像揭示了盐胁迫触发PA积累的动力学和组织特异性。与野生型拟南芥相比，缺失磷脂酶Dα1（PLDα1）的pld α 1突变体的PA积累延迟或减少。对野生型和pld α 1突变体的比较分析表明，细胞pH调节PA与目的蛋白的相互作用以及PLD/PA介导的耐盐性。PA生物传感器PAleon的应用揭示了植物组织中特定的时空PA动力学。我们的研究结果表明，PA信号集成与细胞pH动态介导植物对盐胁迫的反应。
The signalling lipid phosphatidic acid (PA) is involved in regulating various fundamental biological processes in plants. However, the mechanisms of PA action remain poorly understood because currently available methods for monitoring PA fail to determine the precise spatio-temporal dynamics of this messenger in living cells and tissues of plants. Here, we have developed PAleon, a PA-specific optogenetic biosensor that reports the concentration and dynamics of bioactive PA at the plasma membrane based on Förster resonance energy transfer (FRET). PAleon was sensitive enough to monitor physiological concentrations of PA in living cells and to visualize PA dynamics at subcellular resolution in tissues when they were challenged with abscisic acid (ABA) and salt stress. PAleon bioimaging revealed kinetics and tissue specificity of salt stress-triggered PA accumulation. Compared with wild-typeArabidopsis, thepldα1mutant lacking phospholipase Dα1 (PLDα1) for PA generation showed delayed and reduced PA accumulation. Comparative analysis of wild type andpldα1mutant indicated that cellular pH-modulated PA interaction with target proteins and PLD/PA-mediated salt tolerance. Application of the PA biosensor PAleon uncovered specific spatio-temporal PA dynamics in plant tissues. Our findings suggest that PA signalling integrates with cellular pH dynamics to mediate plant response to salt stress.