Mechanisms of RNA recruitment by the exosome
Mechanisms of RNA recruitment by the exosome
复制标题
外泌体招募 RNA 的机制
作者：
H. Malet;E. Lorentzen
外泌体样蛋白复合物是必需的3 ‘ - bbb50 ’核糖核酸酶，参与许多rna的加工和降解。它们在生命的三个领域都是保守的，并共享一个共同的结构，由一个围绕中心通道组织的环状核心结构组成。细菌和古细菌外泌体样复合物降解RNA需要穿过这条单链RNA特异性通道，到达深埋在桶形复合物内的磷酸化活性位点。相比之下，大多数真核外泌体似乎失去了磷酸化活性，而是依赖于水解RNA酶的催化活性，这提出了原核和真核复合物之间RNA募集机制的保护程度的问题。最近对载脂蛋白和RNA结合酵母外泌体的单粒子电镜重建为真核外泌体的通道机制提供了第一个直接的结构证据，表明这种机制在所有的外泌体类复合物之间是保守的。
Exosome-like protein complexes are essential 3’->5’ ribonucleases involved in processing and degradation of many RNAs. They are conserved in the three domains of life and share a common architecture comprised of a ring-like core structure organized around a central channel. RNA degradation by bacterial and archaeal exosome-like complexes requires threading through this single-stranded RNA specific channel to reach the phosphorolytic active sites buried deep within the barrel-shaped complex. In contrast most eukaryotic exosomes appear to have lost phosphorolytic activity and instead rely on hydrolytic RNases for catalytic activity raising the question of the degree of conservation of RNA recruitment mechanisms between prokaryotic and eukaryotic complexes. Recent single particle electron microscopy reconstructions of apo and RNA bound yeast exosomes provide the first direct structural evidence for a channeling mechanism by a eukaryotic exosome suggesting that this mechanism is conserved between all exosome-like complexes.
影响因子：
4.5
作者：
Kiss, Daniel L.;Andrulis, Erik D.
通讯作者：
Andrulis, Erik D.