STUDIES ON IMMUNOCYTOCHEMICAL LOCALIZATION OF INHIBIN-LIKE MATERIAL IN HUMAN PROSTATIC TISSUE - COMPARISON OF ITS DISTRIBUTION IN NORMAL, BENIGN AND MALIGNANT PROSTATES
STUDIES ON IMMUNOCYTOCHEMICAL LOCALIZATION OF INHIBIN-LIKE MATERIAL IN HUMAN PROSTATIC TISSUE - COMPARISON OF ITS DISTRIBUTION IN NORMAL, BENIGN AND MALIGNANT PROSTATES
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DOI：
10.1038/bjc.1986.85
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发表时间：
1986-04-01
影响因子：
8.8
通讯作者：
SHETH, NA
中科院分区：
文献类型：
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作者：
DOCTOR, VM;SHETH, AR;SHETH, NA
针对前列腺来源的类白蛋白物质（ILM）产生了一种特异性抗血清。应用免疫过氧化物酶技术，对114例前列腺组织（正常4例，恶性46例，增生55例）中的ILM进行了定位研究。ILM阳性免疫细胞化学反应仅限于前列腺泡细胞的细胞质，而不是细胞核中的所有三个类别的前列腺，而间质显示阴性反应。阳性反应的强度按以下顺序下降：增生，正常，偶发和中等分化癌，低分化癌，而化生和肉芽肿性前列腺炎ILM阴性反应。使用该实验方案，发现200个非前列腺组织为完全阴性，证明了该试验对前列腺上皮的特异性。这些发现表明ILM作为前列腺组织标记物的潜在用途。
A specific antiserum has been generated against inhibin-like material (ILM) of prostatic origin. Using the immunoperoxidase technique, localization of ILM has been examined in a total of 114 prostates including normal (4 specimens), malignant (46) and hyperplastic (55) tissues. ILM positive immunocytochemical reactions were confined to the cytoplasm and not the nucleus of the prostatic acinar cells in all the three categories of prostate, whereas the stroma showed negative reactions. The intensity of positive reactions decreased in the following order: Hyperplasia, normal, incidental and moderately differentiated carcinomas, poorly differentiated carcinomas, whereas metaplasia and granulomatous prostatitis gave negative reactions for ILM. Using this experimental protocol, 200 non-prostatic tissues were found to be completely negative, demonstrating the specificity of the test for prostatic epithelium. These findings indicate a potential use of ILM as a marker of prostatic tissue.